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Department of Bioengineering

Bioengineering
 

Emily Gibson, PhD

Assistant Professor - Bioengineering


Contact Information

Office: Research 2 - Room 8112
Telephone: 303-724-3678
E-mail: Emily.Gibson@ucdenver.edu

Curriculum Vitae: Download (pdf)

Research Focus

Optical Microscopy and Spectroscopy

Optical microscopy is an area of research focus in my lab. Optical microscopy, in particular fluorescence microscopy, has advanced to a level that allows unprecedented study of biological processes in real-time on the single molecule level. My lab is working on a project to build a Stimulated Emission Depletion (STED) Microscope in order to obtain fluorescent samples at 40-50 nm in resolution as compared to the 200 nm diffraction limit.  With this work, we hope to understand protein-protein interactions.  Spectroscopy is also a focus in my lab.  We use a Raman spectral imager to obtain lipid level information in particular cell and tissue samples for cancer.

  • Integration of Coherent anti-Stokes Raman Spectroscopy (CARS) with microfluidic devices for label-free, biochemical identification in flow cytometry.
  • In vivo detection of changes in extra cellular matrix and cellular metabolism implicated in disease through multiphoton imaging (second harmonic, third harmonic, and autofluorescence lifetime).
  • Development of a STimulated Emission Depletion (STED) microscope for fluorescence imaging of live cells down to resolutions of 25-30 nanometers.

Below are my five most recent published articles. For a complete list, please see my Curriculum Vitae.

1.   M. Potcoava, G.L. Futia, J. Aughenbaugh, I.R. Schlaepfer, E.A. Gibson, “Raman and CARS microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells,” Journal of Biomedical Optics 19(11): 111605 (2014). http://dx.doi.org/10.1117/1.JBO.19.11.111605

2.   O. Masihzadeh, D.A. Ammar, M.Y. Kahook, E.A. Gibson, T.C. Lei, "Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy," Journal Of Biomedical Optics. 2013; 18(3): 036009. http://dx.doi.org/10.1117/1.JBO.18.3.036009

3.  C. Gonzalez-Silva, J. Vera, M.R. Bono, C. González-Billault, B. Baxter, A. Hansen, R. Lopez, E.A. Gibson, D. Restrepo, J. Bacigalupo, "Ca(2+)-Activated Cl(-) Channels of the ClCa Family Express in the Cilia of a Subset of Rat Olfactory Sensory Neurons," PloS One. 2013; 8(7): e69295. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706372

4.   O. Masihzadeh ,T.C. Lei, D.A. Ammar, M. Y. Kahook, E. A. Gibson, “A multiphoton microscope platform for imaging the mouse eye, Mol Vis. 2012; 18:1840-1848. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398498

5.   H. Ma, E.A. Gibson, P.J. Dittmer, R. Jimenez, A.E. Palmer, “High-throughput Examination of FRET-detected Metal-Ion Response in Mammalian Cells,” J. Am. Chem. Soc. 2012; 134 (5): 2488–2491. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297669

University of Colorado Boulder. Post-Doctoral Research Fellow. 2004-2008. Biophysics.
University of Colorado Boulder. PhD. 2004. Physics.
Colorado School of Mines. BS. 1997. Engineering Physics.