Subjects with AUDs will undergo single time point or longitudinal sampling at the University of Colorado and Emory University. Denver is home to a large population of racially and ethnically diverse AUD subjects, including Hispanics, while Atlanta has a sizable African-American population. Denver investigators have enrolled subjects with AUDs through regular screening at Denver Comprehensive Addictions Rehabilitation and Evaluation Services (CARES), while Emory investigators have enrolled subjects at the Atlanta Veteran’s Affairs (VA) Hospital. The majority of subjects with AUDs will undergo a single bronchoscopy, while 30% will have two sequential bronchoscopies performed. The performance of two bronchoscopies in AUD patients will support investigations seeking to establish the persistence of alcohol-related abnormalities. Longitudinal sampling will occur after 1 week of observed alcohol abstinence in an inpatient setting (Denver site), or after 2 or 3 weeks’ outpatient abstinence (Emory site).
Inclusion and exclusion criteria for AUD subjects:
- Inclusion criteria: An Alcohol Use Disorders Identification Test (AUDIT) score of ≥ 8 for men, ≥ 5 for women; last alcohol-containing beverage consumed within the 7 days prior to enrollment; subjects who acknowledge cigarette smoking or cannabis smoking will also be considered.
- Exclusion criteria: history of co-morbidity requiring daily medication (except HTN); concurrent illicit drug use; abnormal chest radiograph or spirometry; age < 18 or > 55.
Clinical protocol. Detailed screening exams will be performed including a full history (demographic, alcohol, tobacco use), physical exam, blood work, chest radiograph, simple spirometry, urine toxicology and cotinine testing, and pregnancy tests (in women). Cigarette and/or cannabis use, if present, will be quantified with validated methods. Abnormal findings (denoted in exclusion criteria) will result in a subject’s exclusion. Subjects will undergo research sampling, including bronchoscopy with BAL and bronchial brushings the morning following admission to ensure active alcohol withdrawal has been addressed if present. Along with subjects who smoke (majority of AUD subjects) active recruitment of AUD subjects who do not smoke either cigarettes or cannabis will also occur to support focused investigations of AUDs.