Lab Personnel

David Costantino, M.S., Lab Manager and Senior Professional Research Assistant

david.costantino_at_cuanschutz.edu

”In my role as lab manager I am responsible for much of the day to day operation, but I also get to do research, which is the main reason I am so excited to be part of this group. When people ask me what I do for a living, I tell them I can save the crickets, and that is always a great way to introduce what we study in our lab.”

David likes donuts…with sprinkles. He also plays tennis, eats BBQ and Italian food, loves baseball, plays poker, goes to lots of hockey games, and teaches chess to school kids and adults. Scouting reports describe him as a fantasy sport force to be reckoned with whenever he steps on the field.

Andrea MacFadden, Professional Research Assistant

andrea.macfadden_at_cuanschutz.edu

"I am studying the effects of how the xrRNAs within the 3' UTRs of flaviviruses affect degradation by a variety of ribonucleases. I am hoping this will allow us to crystallize these diverse xrRNAs to discover their structures, 'cause that's what we do in the Kieft lab."

Andrea joined the lab with lots of experience expressing and purifying proteins. We have converted her to the RNA world...although she is still a Redwings fan...we have to work on that.

Parker Nichols, Graduate Student (Structural Biology and Biochemistry). Parker is a joint student with the Vogeli lab

parker.nichols_at_cuanschutz.edu

"I am excited to study how RNA structure and dynamics dictates function as a new addition to both the Kieft and Vogeli labs. Specifically, I am working on understanding how the RNA binding domains of human ADAR1 recognize unique RNA conformations in order to regulate the cellular immune response and how Xrn1-resistant RNA folding dynamics plays a role in inhibiting degradation by exonuclease Xrn1. These questions are critical in understanding the complex interactions between viruses and their hosts."

Parker grew up in Oregon, and spent a lot of time in Portland. Therefore, he is a fan of microbreweries and good restaurants, as long as they are not too popular... He is passionate about science, especially structural biology, and he aspires to become a master of all three structural techniques. In his free time, he likes to hike and play tennis

Conner Langeberg, Graduate Student (Structural Biology and Biochemistry)

conner.langeberg_at_cuanschutz.edu

"I am currently working to biochemically and structurally characterize a class of viral, histidine accepting tRNA-like structures. These structures are functional mimics of host tRNAs and participate in the co-opting of host cell machinery for vial replication. I am using crystallography and cryo-EM to determine the structure of these RNAs alone and in complex with the host histidine synthetase and CCA-NTase, and the viral RdRp to better understand how these structures are recognized and acted upon."

As a #ColoradoNative, Conner enjoys many forms of outdoor activities, including, but not limited to, hiking, camping, skiing, snowshoeing, whitewater rafting, paddleboarding, and attempting to combine the aforementioned activities with food and his dogs.

Rachel Jones, Graduate Student (Molecular Biology)

rachel.a.jones_at_cuanschutz.edu

“I am excited to continue learning about RNA and RNA-related disease in the Kieft Lab. My current scientific interests lie in functionally and structurally characterizing a distinct set of non-coding RNA molecules belonging to flaviviruses. The objective of my project is to determine the structure of these RNA molecules in distantly related viruses and eventually understand the evolution of these RNA structures. As my studies progress, I plan to study other RNA molecules that play roles in high-mortality diseases.”

Like any other young person, Rachel's hobbies include bird watching, arts and crafts, and caring for her cats and plants. Although her dream job was to be a zoo vet, she is pretty happy with the route my career has taken. Her not-so-guilty pleasure is Cookies n’ Cream ice cream, which she considers part of a healthy, balanced diet.

Matthew Szucs, Graduate Student (Microbiology). Currently funded by the Molecular Pathogenesis of Infectious Disease T32 training grant.

matthew.szucs_at_cuanschutz.edu

"I am excited to join the Kieft lab as a microbiology graduate student. My current interests involve searching for and identifying homologs of viral xrRNA, or other interesting structural motifs in other microscopic life. I plan on combining computational methods as well as biochemical and molecular techniques address this this question. I am also incredibly eager to dive into the wonderful world of RNA structure and function."

As a former east coast resident, Matt is excited to continue his scientific career in Colorado. His interests outside of lab include copious amounts of crafting, cooking elaborate meals, birds, coffee, and music. His ideal evening: A long walk on the beach with his cat Junipur.

Zoe O'Donoghue, Graduate Student (Microbiology), currently an RNA BioScience Initiative Scholar

zoe.odonoghue_at_cuanschutz.edu

"I am now the most senior graduate student in the lab and I’m still excited to be exploring the RNA structure/function relationship of flaviviral 3'UTRs. While I like all the flaviviruses equally, I have chosen the path of being the only person on campus to work with Dengue virus, which has led to a pretty steep learning curve but I think some pretty cool discoveries as well. I am combining viral infection models with structural studies to understand various mechanisms of Dengue pathogenicity and infection dynamics. Basically, I plan to save the world."

Zoe fits every Colorado stereotype - she has a dog, drives a Subaru, has a profound appreciation for microbrews, is a devout Broncos fan, and loves to ski/snowboard. Her other hobbies include but are not limited to: daydreaming, scuba diving, binge reading/watching/eating, and any kind of exercise to balance out the binges.

Ben Akiyama, PhD., Postdoctoral Fellow (Twitter: @BenAkiyama)

benjamin.akiyama_at_cuanschutz.edu

“I am currently studying how the dynamics of structured viral RNA elements impact their function. This includes investigating how the folding and unfolding pathways of viral RNAs affect their unique properties and studying the motions of various RNA domains as they interact with cellular machinery. By breaking down these processes, I hope to be able to create a ‘movie’ of these important molecules in action.”

Ben comes with a background in single-molecule fluorescence of RNA-protein complexes. He grew up in San Francisco, which makes him a 49ers and Giants (the baseball kind) fan. We like him anyway (at least every other year...).

Daniel Eiler, PhD., Postdoctoral Fellow

daniel.eiler_at_cuanschutz.edu

”My interests are to use biochemical and crystallographic approaches to characterize the interactions between a viral RNA and its cellular protein target, and to understand how this interaction manipulates the protein’s function. Specifically, I want to find out how the RNA element recognizes its target, elucidate the important interactions between the two molecules, and understand how this induces a certain viral outcome.”

Daniel brings a wealth of structural knowledge and experience. He likes running, lifting, football, baseball, board games, camping, swing dancing, and traveling. His favorite TV shows are The Big Bang Theory and Elementary.

Maddie Sherlock, Jane Coffin Childs Postdoctoral Fellow (Twitter: @madisherlikin!)

madeline.sherlock_at_cuanschutz.edu

"My current research is exploring the structural diversity within a class (type III) of IRES RNAs. While IRES RNAs can generally be categorized by which initiation factors they require for translation to occur, type III IRES RNA representatives from different viruses display variable translation efficiencies depending on the cell type or stress conditions. I am further investigating these observations by identifying and structurally characterizing additional IRES RNA-protein interactions."

Maddie is an RNA enthusiast making the switch from studying bacterial ncRNAs to viral ncRNAs. She has two cats and a dog to bring along on her many brewery visits and hiking trips. Now her assimilation into Colorado culture is complete! She loves traveling, cooking, playing softball, and spinning classes. Follow her on twitter: @madisherlikin!

Steve Bonilla, PhD., Postdoctoral Fellow (Twitter: @Steve_Bonilla)

steve.bonillarosales_at_cuanschutz.edu

“I am using single-particle cryogenic electron microscopy (cryo-EM), biochemistry, and other experimental and computational tools to dissect RNA structures and dynamics. Ultimately, I want to reveal generalizable principles that dictate the diverse and dynamic conformational landscape of functional RNAs”

Steve was born and raised in Guatemala. He obtained his BS in chemical engineering from University of Washington and his PhD in chemical engineering from Stanford University. Steve’s PhD dissertation focused on single-molecule and high-throughput studies of RNA 3D folding thermodynamics. In his free time, Steve likes to play the guitar, write music, read, and travel. Steve is also working hard towards acquiring the essential set of ‘outdoorsy’ skills necessary to (socially) survive in Denver.

Anna-Lena Steckelberg, PhD., Postdoctoral Fellow (Twitter: @LenaSteckelberg)

anna-lena.steckelberg_at_cuanschutz.edu

"I study a viral RNA element that inhibits cellular exoribonucleases. Using a combination of biochemical assays, x-ray crystallography and single molecule FRET, I aim to elucidate how the dynamic folding of this RNA structure impedes protein function in a process that leads to the generation of viral long non-coding RNAs."

Lena moved from the University of Cologne to Denver to broaden her RNA world and is fully Coloradoan. She likes to hike, climb, camp, and ski, and she is pleasantly surprised by the large selection of beer in Colorado's pubs. But....she still does not understand American sports (especially baseball). Lena, it is easy: you catch the ball, you throw the ball, you hit the ball....and don't lollygag.

Quentin Vicens, PhD., Research Assistant Professor (Twitter: @qvicens)

quentin.vicens_at_cuanschutz.edu

"RNA never stops amazing us with intricate folds that subtly support regulatory functions. Viral RNA structures represent a fantastic opportunity to continue capturing glimpses of how RNA operates, with relevance to biomedicine."

Quentin is a seasoned bushwhacker of the RNA jungle, having resorted to various structural and molecular biology techniques for challenging anything ribo (ribozyme, riboswitch, ribosome, ...). He is passionate about the educational process, likes to read and watch movies, cook and eat great food, talk for hours as if on his favorite French TV book show, and spend time with his family, especially outdoors.

Brian Wimberly, PhD., Research Assistant Professor

brian.wimberly_at_cuanschutz.edu

Brian wants to push the frontiers of structural biology. Beyond that, you aren’t getting any more information out of him, so don’t even try.

University of Colorado Department of Biochemistry and Molecular Genetics