I study chromatin structure and function at a global level using high throughput sequencing technology. I am studying the chromatin structure through superhelical density across the genome in wild type cells as well as looking at the interplay between nucleosome structure and chromatin structure by looking at histone mutations that disrupt the core nucleosomal structure. To understand how modifications to the histones regulate the positioning of nucleosomes and the structure of the chromatin, I am investigating the role of 4 histone modifications in the nucleosome core; histone H3 lysine (K)56 acetylation, K115 acetylation, threonine (T)118 phosphorylation, and K122 acetylation to determine the affect these modifications have on global nucleosome positioning in yeast.
I obtained a Ph.D. From the University of Colorado at Boulder studying the role of phospholipid flippases on programmed cell death in C. elegans.
Ransom M, Dennehey BK, Tyler JK (2010) Chaperoning Histones during DNA Replication and Repair. Cell, 140: 183–195.
Ransom M, Williams S, Mekonnen X, Das C, Linger J, Adkins M, Liu C, Bartholomew B, Tyler JK. (2009) FACT and the proteosome promote promoter nucleosome disassembly and transcriptional initiation. J Biol Chem, 284, 23461.
Darland-Ransom M Wu YC, Xue D. Programmed cell death in C. elegans. Edited by X.M.Yin and Z. Dong. The Humana Press Inc., 2009, 355-373.
Darland-Ransom M Wang X, Sun C, Mapes J, Gengyo-Ando K, Mitani S, Xue D. (2008). C. elegans TAT-1 protein is important for maintaining plasma membrane phosphatidylserine asymmetry. Science, 320, 528.
Fay DS, Large E, Han M, Darland M. (2003). lin-35/Rb and ubc-18, an E2 ubiquitin-conjugating enzyme, function redundantly to control pharyngeal morphogenesis in C. elegans. Development, 130, 3319.
Hanekamp T, Thorsness MK, Rebbapragada I, Fisher EM, Seebart C, Darland MR, Coxbill JA, Updike DL, Thorsness PE. (2002). Maintenance of mitochondrial morphology is linked to maintenance of the mitochondrial genome in Saccharomyces cerevisiae. Genetics 162, 1147.