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Services


 Flow Cytometry Analysis

BD FACS Calibur
  • 2 Lasers: 488 and 640
  • 4 color analysis
  • Suggested Fluors: FITC, PE, PerCP, and APC
  • Configuration​
BD FACS LSRFortessa
  • 5 lasers: 355, 405, 488, 561, and 640
  • 17 color analysis
  • Configuration
BC CytoFlex S
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 Cell Sorting

BD FACSAria Fusion
  • 70um, 85um, and 100um nozzle tips
  • 4 Lasers: 405, 488, 561, 640
  • Biosafety cabinet for sorting human cells safely
  • 4-way and plate sorting
  • Operator and self-run options available
  • Configuration

To optimize samples for cell sorting it is suggested to include the following in your reagents to bring to the core:
  1. Basic Sorting Buffer
    • ​​1X Hanks Balanced Salt Solution (HBSS) or Phosphate Buffered Saline (PBS) Both phenol red and Ca/Mg++ free
    • 1mM EDTA
    • 25mM HEPES pH 7.0
    • 1% FBS or BSA (BSA is recommended when using EDTA)
    • 0.2um sterile filter, store at 4 degrees C
  2. For samples with a h​igh percentage of dead cells: Add 25-50ug/ml of DNAse I (Cat# D4513, Sigma-Aldrich Co.) and 5mM MgCl2 (Magnesium chloride hexahydrate, Cat# M2670, Sigma-Aldrich Co.) to the buffer.
  3. Viability Dye
  4. Tubes - Polypropylene
  5. Samples must be filtered​ prior to sorting (Falcon Ref 352235)
  6. Basic sorting Buffer, FBS or media to sort into
Facility can only sort BSL2

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 CyTOF Reagents

Please use the following form and email it to erin.kitten@ucdenver.edu


The core provides the following reagents:
  • Fluidigm conjugated mouse and human antibodies
  • ​​Self-conjugated mouse and human antibodies
  • MarxPar Barcoding kits
  • Cisplatin and Intercalator
  • MaxPar Antibody Conjugation Kits with metals
  • Fluidigm PBS, Cell Staining Media, 5X Fix Buffer​
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