Skip to main content
Sign In


 Flow Cytometry Analysis

BD FACS Calibur
  • 2 Lasers: 488 and 640
  • 4 color analysis
  • Suggested Fluors: FITC, PE, PerCP, and APC
  • Configuration​
  • 5 lasers: 355, 405, 488, 561, and 640
  • 17 color analysis
  • Configuration
BC CytoFlex S

 Cell Sorting

BD FACSAria Fusion
  • 70um, 85um, and 100um nozzle tips
  • 4 Lasers: 405, 488, 561, 640
  • Biosafety cabinet for sorting human cells safely
  • 4-way and plate sorting
  • Operator and self-run options available
  • Configuration

To optimize samples for cell sorting it is suggested to include the following in your reagents to bring to the core:
  1. Basic Sorting Buffer
    • ​​1X Hanks Balanced Salt Solution (HBSS) or Phosphate Buffered Saline (PBS) Both phenol red and Ca/Mg++ free
    • 1mM EDTA
    • 25mM HEPES pH 7.0
    • 1% FBS or BSA (BSA is recommended when using EDTA)
    • 0.2um sterile filter, store at 4 degrees C
  2. For samples with a h​igh percentage of dead cells: Add 25-50ug/ml of DNAse I (Cat# D4513, Sigma-Aldrich Co.) and 5mM MgCl2 (Magnesium chloride hexahydrate, Cat# M2670, Sigma-Aldrich Co.) to the buffer.
  3. Viability Dye
  4. Tubes - Polypropylene
  5. Samples must be filtered​ prior to sorting (Falcon Ref 352235)
  6. Basic sorting Buffer, FBS or media to sort into
Facility can only sort BSL2


 CyTOF Reagents

Please use the following form and email it to

The core provides the following reagents:
  • Fluidigm conjugated mouse and human antibodies
  • ​​Self-conjugated mouse and human antibodies
  • MarxPar Barcoding kits
  • Cisplatin and Intercalator
  • MaxPar Antibody Conjugation Kits with metals
  • Fluidigm PBS, Cell Staining Media, 5X Fix Buffer​