The UC Denver Department of Immunology and Microbiology Flow Cytometry Shared Resource Laboratory (SRL) is dedicated to the education and training, both in theory and practice, of the researchers utilizing the SRL by providing state of the art instrumentation, expertise and consultation. The SRL is committed to providing high quality assurance of instrumentation, data quality and appropriate interrogation of data.
The SRL is under the direction of Laurent Gapin, Ph.D. and the management of Erin Kitten, BS.
Equipped with three flow cytometers (BDFACS LSRFortessa, BDFACS Calibur, BC CytoFlex S), a cell sorter (FACSAria Fusion) and reagent banks for CyTOF (antibodies, barcoding kits and antibody labeling reagents) the SRL is dedicated to providing the resources necessary for investigators with a primary appointment in the Department of Immunology and Microbiology to be leaders in their respective fields. Investigators with secondary appointments or those that are affiliated faculty and wish to utilize the services and resources provided by the Flow Cytometry SRL, please contact Laurent Gapin, PhD (Laurent.Gapin@ucdenver.edu), Flow Cytometry SRL Scientific Director, to discuss your options or Kimberly Jordan (Kimberly.Jordan@ucdenver.edu), Assistant Director of the HI3 Human Immune Monitoring Shared Resource (HIMSR), to partner with the HIMSR to assist with your cytometry research needs.
- Service - To provide access to investigators 24 hours to both state-of-the-art flow cytometers and a cell sorter and to assist with experimental design and assay development.
- Education – To develop and provide educational and training opportunities for new and experienced researchers. Seminars will introduce attendees to the foundations of instrumentation and experimentation as well as expand their knowledge of emerging technologies.
- Research – To provide a rigorous quality assurance schedule that yields the highest quality of data that is dependable, consistent and reproducible. Partnership with the Human Immunology and Immunotherapy Initiative (HI3) Human Immune Monitoring Shared Resource (HIMSR) will provide continuous standardization of new techniques and applications in the context of flow cytometry and mass cytometry (CyTOF).
Please acknowledge the Department of Immunology and Microbiology Flow Cytometry SRL in all publications and grant applications where equipment use and/or services and/or expertise contributed to the final work product. One example of an acknowledgment is as follows: “This work was supported by the UC Denver Immunology and Microbiology Flow Cytometry SRL.” Publication co-authorship should be considered when a significant contribution to the experimental design or final outcome of the study was provided by the Flow Cytometry SRL.
Please send Laurent Gapin (Laurent.Gapin@ucdenver.edu) a PDF of all publications that contain data generated from the SRL. Additionally, please email Laurent Gapin if you were awarded a grant that contains data generated from the SRL.
The Department of Immunology and Microbiology Flow Cytometry SRL sincerely thanks the Human Immunology and Immunotherapy Initiative (HI3) and the HI3 HIMSR for continued support.