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Molecular Genetics Analysis Core

Director: Dr. Richard Spritz


Consultation. The SDRC Molecular Genetic Analysis Core provides expertise and advice to UCAMC-SDRC investigators in design and analysis of many different types of studies that require molecular genetic analyses. UCAMC-SDRC investigators are not charged for this service.

DNA Preparation and QC. Many different types of molecular genetic analyses require preparation of high-quality genomic DNA. The SDRC Molecular Genetic Analysis Core provides preparation of genomic DNA from blood, tissue, or saliva, involving small to very large numbers of samples. DNA concentrations and amounts are determined by Qubit fluorometry or Nanodrop spectrophotometry, and DNA can be provided to users in Eppendorf tubes in either TE or water, as specified by the user.

Genotyping. The SDRC Molecular Genetic Analysis Core provides or facilitates access to smaller-scale to larger-scale (to genomewide), user-defined genotyping of polymorphic DNA markers, particularly single-nucleotide polymorphisms (SNPs) as appropriate to project needs. Small-scale genotyping is carried out for 1-48 SNPs in up to 1000 individuals. Intermediate-scale genotyping can be carried out for 96-1536 SNPs in 12 to thousands of individuals. Large-scale genotyping can be carried out for up to millions of SNPs in up to thousands of individuals. Genotyping of human or mouse SNPs can be provided as needed. The core provides free consultation in study design, data QC, and post-analysis for small-scale projects. The core can also provide data-warehousing for intermediate-scale and large-scale projects.

NA Sequencing.NA Sequencing.NA Sequencing.NA Sequencing. The SDRC Molecular Genetic Analysis Core provides or facilitates access to smaller-scale (Sanger) to larger-scale (Next-Generation targeted or whole-exome) DNA sequencing analysis and interpretation, as appropriate to member project needs.

RNA Expression Analysis. The SDRC Molecular Genetic Analysis Core facilitates access to small-scale quantitative RNA expression assay by qPCR, for 1-30 user-defined transcripts across up to 384 samples. Microarray-based and RNA-seq based assays are also available by arrangement.

Contacts:

Dr. Richard Spritz: richard.spritz@ucdenver.edu

Dr. Tracey Ferrara: tracey.ferrara@ucdenver.edu