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Welcome to the University of Colorado School of Medicine on the Anschutz Medical Campus. We are a Top Ten Medical School in primary care, pediatrics, family medicine and rural medicine, and offer physical therapy and physician assistant programs.

The Graduate School at UC Denver

Peptide Protein Chemistry Facility

The Peptide and Protein Chemistry Facility at the University of Colorado at the Anschutz Medical campus provides peptide synthesis, purification and peptide/protein composition analysis as well as mass spectrometry services for academic and industrial clients.

The Peptide and Protein Chemistry facility personnel help users choose and design experiments using appropriate chemistry/instrumentation, to obtain the necessary data, and interpret the results. The Facility also provides instruction for graduate students and postdoctoral fellows for the use of HPLC and LC/MS approaches.

The facility is equipped with state of the art instrumentation and technical support suitable for diverse applications and samples.

Beckman 6300 AA analyzer

Agilent 1100 HPLC systems

Agilent SL/XCT Electrospray MS systems

Advanced Chemtech peptide synthesizer


  • Minimum charge is for 10 residues ($250)
  • 0.1 mM scale (50-100 mg crude peptide): $25 per residue
  • 0.25 mM scale (100-300 mg crude peptide): $40 per residue
  • All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and mass spectrometry
  • Preparative HPLC purification of synthetic peptides: $100 per peptide
  • Additional modification of peptides:
    • phosphorylation: $100 - $150
    • acetylation: $15
    • C-terminal biotin: $175
    • fluorescent label: $200
    • N-terminal biotin: $100
    • methylation: $175 - $400


  • $20 per sample


  • Mass determination of proteins, peptides, etc: $15 per sample, $100/hour
  • LC/MS/MS analysis: $30 per sample, $100/hour
  • 2D LC/MS/MS analysis: $100/sample
  • Enzymatic digestion of proteins in gel slices and protein identification byLC/MS/MS: $200 - $500

Contact Information

Peptide and Protein Chemistry Facility 
University of Colorado Anschutz Medical Campus 
Bldg. RC-1 South, Room L18-1308 
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045

Dr. Robert S. Hodges, Director
voice: 303-903-5591
fax: 303-724-3249
Office: Fitzsimons, RC-1 South Tower, Room 9121

Dziuleta Cepeniene, Professional Research Assistant
voice: 303-724-3336
Office: Fitzsimons, RC-1 South Tower, Room 1308


Elizabeth Wethington, Program Administrator
Voice: 303-724-7280 | Email:
12800 E. 19th Avenue, Mail Stop 8303, Research Complex 1 South Tower, Room 6129, Aurora, CO 80045

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