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Peptide Protein Chemistry Facility


The Peptide and Protein Chemistry Core Facility at the University of Colorado, Anschutz Medical Campus provides state-of-the-art instrumentation for solid-phase peptide synthesis using Boc- and Fmoc-chemistry, peptide purification by rev​ersed-phase HPLC and peptide identification by mass spectrometry (LC-MS) services for academic and industrial clients.


Core Facility personnel will help users select and design the appropriate peptide sequences for their applications including how and where to incorporate fluorescent and biotin labeled probes, incorporation of modified and isotopic labeled amino acids and covalent constraints to stabilize the peptide conformation, etc.






Automated Solid-Phase Peptide Synthesizers

AAPPTec Focus XC-4RV for Boc- and Fmoc-chemistry

CEM Liberty Microwave for Fmoc-chemistry


Analytical and Preparative HPLC Systems

Agilent 1100 and 1200 HPLC analytical systems

Agilent 1100 LC/Electrospray MS system

Beckman System Gold preparative HPLC system (10 ml/min max.)

Sharp Semi-prep Gradient Purification System (5 to 50 ml/min)


HF Cleavage apparatus for Boc-chemistry


Peptide Synthesis (Solid-phase / solution phase)

Peptides can be synthesized using L- and/or D-amino acids or unusual amino acids. Boc-, Fmoc- or a combination of these synthesis chemistries can be used.

Peptide Purification (Reversed-phase HPLC)

Our facility only provides synthetic peptides at purities greater than 95% at the milligram to gram scale. All peptides are analyzed for purity by analytical reversed-phase HPLC and characterized by LC-MS (electrospray mass spectrometry).

Peptide Characterization (LC/MS)

Peptide Modification

See the rates and policies tab for possible modifications. If you don't see the modificiation you want, feel free to inquire.

Peptide Conjugation (polyclonal/monoclonal antibody production)

We can couple synthetic peptides to carrier proteins for production of polyclonal or monoclonal antibodies. We generally couple to Keyhole limpet hemocyanin (KLH) for antibody production and Bovine serum albumin (BSA) for screening antibodies by ELISA.

Quantitation (amino acid analysis)


PEPTIDE SYNTHESIS

Our staff has over 100 years of experience in peptide design and synthesis, and we are happy to discuss your order with you at no charge. Customers will receive a quotation prior to beginning production to assess costs. The costs of synthesis are dependent on peptide length and the incorporated modifications. Some bulk discounts may be available depending on the project.

  • Minimum charge for synthesis ($250)
  • 0.1 mM scale: $25 per residue
  • 0.25 mM scale: $40 per residue
  • All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and LC-MS
  • Preparative HPLC purification of synthetic peptides: $250 per peptide (depending on scale of synthesis)
  • Additional modification of peptides:
    • Incorporation of phosphorylated Ser or Thr
    • Incorporation of D-amino acids or other unusual amino acids
    • Acetylation
    • Biotin (C-terminal or N-terminal)
    • Fluorescent label
    • Conjugation to KLH or BSA
    • Methylation

AMINO ACID ANALYSIS

  • $40 per sample

LC/MASS SPECTROMETRY

  • LC-Mass determination of proteins, peptides, etc: $15 per sample

Contact Information

Peptide and Protein Chemistry Facility 
University of Colorado Anschutz Medical Campus 
Bldg. RC-1 South, Room L18-1308 
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045

Dr. Robert S. Hodges, Director
Phone: 303-903-5591
Fax: 303-724-3249
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L9121
Robert.Hodges@ucdenver.edu

Dr. Lajos Gera, Research Associate Professor
Phone: 303-724-3336
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308
Lajos.Gera@ucdenver.edu

Timothy Davis, Professional Research Assistant
Phone: 303-724-3336
Office: UCDenver - Anschutz Medical Campus, Bldg. RC-1S, Rm. L1308
Timothy.Davis@ucdenver.edu

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