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Peptide Protein Chemistry Facility

​   The Peptide and Protein Chemistry Core Facility at the University of Colorado, Anschutz Medical Campus provides state-of-the-art instrumentation for solid-phase peptide synthesis using Boc- and Fmoc-chemistry, peptide purification by rev​ersed-phase HPLC and peptide identification by mass spectrometry (LC-MS) services for academic and industrial clients.

Core Facility personnel will help users select and design the appropriate peptide sequences for their applications including how and where to incorporate fluorescent and biotin labeled probes, incorporation of modified and isotopic labeled amino acids and covalent constraints to stabilize the peptide conformation, etc.

Automated Solid-Phase Peptide Synthesizers

AAPPTec Focus XC-4RV for Boc- and Fmoc-chemistry

CEM Liberty Microwave for Fmoc-chemistry

Analytical and Preparative HPLC Systems

Agilent 1100 and 1200 HPLC analytical systems

Agilent 1100 LC/Electrospray MS system

Beckman System Gold preparative HPLC system (10 ml/min max.)

Sharp Semi-prep Gradient Purification System (5 to 50 ml/min)

HF Cleavage apparatus for Boc-chemistry

Peptide Synthesis (Solid-phase / solution phase)

Peptide Purification (Reversed-phase HPLC)

Peptide Characterization (LC/MS)

Peptide Modification

Peptide Conjugation (polyclonal/monoclonal antibody production)

Quantitation (amino acid analysis)

1. The key to successful peptide research projects is consultation between the researcher and core facility staff.

2. We will help select and design the appropriate peptide sequence for each application including how and where to incorporate fluorescent and biotin labelled probes, incorporation of modified and isotopic labelled amino acids and covalent constraints to stabilize peptide conformation, etc.

3. Prices for synthetic peptides are dependent on peptide length and quantity required by the researcher.

Our prices are competitive with all other facilities world-wide.

No Charge for Consultation.

4. Our facility only provides synthetic peptides at purities greater than 95% at the milligram to gram scale.

5. All peptides are analyzed for purity by analytical reversed-phase HPLC and characterized by LC-MS (electrospray mass spectrometry).

6. Quotations are provided for each peptide project and cost reductions are given for ordering large numbers of peptides.

7. Peptides can be synthesized using L- and/or D-amino acids or unusual amino acids.

8. Depending of the requirement of the researcher peptides are synthesized using Boc-, Fmoc- or a combination of these chemistries.

9. We can couple synthetic peptides to carrier proteins for production of polyclonal or monoclonal antibodies. We generally couple to Keyhole limpet hemocyanin (KLH) for antibody production and Bovine serum albumin (BSA) for screening antibodies by ELISA.


  • Minimum charge is for 10 residues ($250)
  • 0.1 mM scale (50-100 mg crude peptide): $25 per residue
  • 0.25 mM scale (100-300 mg crude peptide): $40 per residue
  • All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and mass spectrometry
  • Preparative HPLC purification of synthetic peptides: $100 per peptide
  • Additional modification of peptides:
    • phosphorylation: $100 - $150
    • acetylation: $15
    • C-terminal biotin: $175
    • fluorescent label: $200
    • N-terminal biotin: $100
    • methylation: $175 - $400


  • $20 per sample


  • Mass determination of proteins, peptides, etc: $15 per sample, $100/hour
  • LC/MS/MS analysis: $30 per sample, $100/hour
  • 2D LC/MS/MS analysis: $100/sample
  • Enzymatic digestion of proteins in gel slices and protein identification byLC/MS/MS: $200 - $500

Contact Information

Peptide and Protein Chemistry Facility 
University of Colorado Anschutz Medical Campus 
Bldg. RC-1 South, Room L18-1308 
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045

Dr. Robert S. Hodges, Director
voice: 303-903-5591
fax: 303-724-3249
Office: Fitzsimons, RC-1 South Tower, Room 9121

Dziuleta Cepeniene, Professional Research Assistant
voice: 303-724-3336
Office: Fitzsimons, RC-1 South Tower, Room 1308


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