The goal of the UC Denver School of Medicine's Biological Mass Spectrometry Facility is to provide investigators with the capabilities to identify, characterize, and quantify biomolecules present in tissues, cells, and biological fluids. The facility houses both Proteomics and Metabolomics Cores, and aims to assist members with solving difficult or previously intractable problems in biomedical research. Methods for biomolecule isolation, separation, quantification, identification and bioinformatics analysis, together with expert guidance in study design, are integrated into expertise offered by the Facility. Our access to diverse analytical technologies thereby allows investigators to adopt multiple strategies and to independently verify their findings. The Biological MS Facility also provides training in proteomic and metabolomic analysis and experimental design.
The Facility participates in a series of short courses relating to aspects of mass spectrometry and proteomics. Both lecture-based and hands-on courses are available, and these are open to outside participants. If you are interested in attending any of these please email Dr. Kirk Hansen
Work performed in or by the Biological MS Facility should be acknowledged in presentation and/or publication. We ask that a reprint of publications and presentations that include data obtained in or by the facility be retained for our records. This allows us to document activities and aids in gaining funding to maintain and upgrade core equipment.
The Association of Biomolecular Resource Facilities (ABRF) sponsors yearly studies to assist research labs and cores evaluate their capabilities to identify and characterize "unknown" biological samples. The goal is to evaluate the technologies and educate their members. Results of past studies and information on more recent studies are posted on their site. Our Facility actively participates in proteomic and protein sequencing studies, with previous studies aiming to address groups' abilities to identify proteins in a simple mixture, identification of phosphorylation sites, discrimination of protein isoforms, and accuracy of de novo peptide sequencing.
The facility is a collaborative research resource that balances applied MS-based "omics" research with the development of new and improved methods for the identification and quantification of proteins, peptides, and metabolites in a multitude of complex systems. The facility encourages collaborations that apply the tools of proteomics and/or metabolomics to biomedical research. Prospective users are encouraged to contact Dr. Monika Dzieciatkowska, Dr. Angelo D'Alessandro or Dr. Kirk Hansen.
The Proteomics and Metabolomics Cores are housed within the School of Medicine Biological Mass Spectrometry Facility on the ground floor of RC-1 South at the Anschutz Medical Campus. (Room L18-1303)