The UC Denver Biological Mass Spectrometry Facility is equipped with the instrumentation and expertise to perform detailed proteomic and metabolomic studies. Major instrumentation in the facility include the following:
Thermo Scientific LTQ Orbitrap Velos Pro,
hybrid linear ion trap-Orbitrap high resolution mass spectrometer. This
instrument is used for proteomic applications, including protein
identification and detailed peptide mapping. Identification of proteins
is performed on proteolytic digests by means of nanoflow LC-ESI-MS/MS
with subsequent database searches using software packages such as Mascot
and Protein Prospector. This instrument is capable of electron
transfer dissociation (ETD) which is useful for analysis of many
post-translational modifications. This instrument was funded by the
National Institutes of Health-National Center for Research Resources
Thermo Scientific Q Exactive, a hybrid quadrupole-Orbitrap high resolution mass spectrometer. This instrument offers rapid scanning, high resolving power (240,000 at m/z 200), facile polarity switching, and high-energy collision dissociation (HCD). The Q Exactive is extremely versatile and used in a wide variety of survey and targeted metabolomic and proteomic applications. Inquire for availability.
GE Healthcare, Life Sciences TyphoonTM Variable Mode Imager featured with versatile system platform, handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays. Consists with powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets. Red, green, and blue excitation wavelengths and a wide shoice of emission filters enable imaging of an extensive variety of fluorophores. Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.