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Faculty & Staff Directory

Marc Goalstone, Ph.D.


Dr. Marc Goalstone

Lecturer

Email:  Marc Goalstone
Office Location: SI 4087
Phone:  (303) 352-3794
Fax:  (303) 556-4352
Office hours:  T 6:30p-7:30p
Areas of Expertise:
Endocrinology

Education & Degrees 

Ph.D., University of Denver, Denver, Colorado, June 1995, Cell Biology, Dissertation title: “Characterization of Farnesyltransferase in Xenopus laevis Oocytes”.

  

M.A., University of Colorado at Denver, Denver, Colorado, August 1992, Major: Cell Biology. Master’s Thesis title: The effect of Microinjected ras p21 on Inositol 1,4,5-trisphosphate and sn 1,2-diacylglycerol Mass in Xenopus.

 

Associates degree in Law, Denver Paralegal Institute, Denver, Colorado, May 1979: Paralegal, Fogel, Keating and Wagner Law Firm.

 

B.A., The Colorado College, Colorado Springs, Colorado, June 1974, Major: Biology.

​Bio

Dr. Goalstone’s lab is focused on insulin signaling as it relates to obesity, cardiovascular and neurodegenerative diseases. In particular, he is determining intracellular mechanisms that highlight the pathophysiological dysfunction of intracellular insulin signaling as the sequelae of hyperinsulinemia and insulin resistance in the context of Type-2 Diabetes Mellitus.

 

​Select Publications 

Stith, Bradley, J., Kirkwood, Allan J., and Goalstone, Marc L. (1992). Protein Kinase C initially inhibits the induction of meiotic cell division in Xenopus oocytes. Cellular Signaling

4(4):393-403.

 

Stith, Bradley, J., Jaynes, Chris, Goalstone, Marc L., and Silva, Sally. (1992). Insulin and progesterone increase 32-PO4-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in Xenopus oocytes. Cell Calcium 13:341-352.

 

Stith, B.J., Silva, S., Jaynes, C., and Goalstone, M.L. (1992). Inositol 1,4,5-trisphosphate and diacylglycerol increase from fertilization through first cleavage in Xenopus laevis. Mol. Biol. Cell 4(4):435-442.

 

Goalstone, M.L. , and Sadler, S.E., (1996). Characterization of Xenopus laevis oocyte farnesyltransferase. Biol. Reprod. 54:675-681.

 

Stith, B.J., Goalstone, M.L., Espinoza, R., Mossel, C., Roberts, D., and Wiernsperger, N. (1996). The anti-diabetic metformin elevates receptor tyrosine kinase activity and inositol 1,4,5-trisphosphate mass in Xenopus oocytes. Endocrinology 137(7):2990-2999.

 

Goalstone, M.L., and Draznin, B., (1996). Effect of insulin on farnesyltransferase activity in 3T3-L1 adipocytes. J. Biol. Chem.  271(44):27585-27589.

 

Leitner, J.W., Kline, T., Carel, K., Goalstone, M., and Draznin, B. (1997). Hyperinsulinemia potentiates activation of p21Ras by growth factors. Endocrinology 138(5):2211-2214.

 

Goalstone, M.L., and Draznin, B. (1997). Insulin Signaling. West. J. Med. 167:166-173.

 

Goalstone, M., Carel, K., Leitner, J.W., and Draznin, B. (1997).  Insulin stimulates the phosphorylation and activity of farnesyltransferase via the Ras-MAP kinase pathway. Endocrinology 138:5119-5124.

 

Goalstone, M.L., Leitner, J.W., and Draznin, B. (1997) GTP loading of farnesylated p21Ras by insulin at the plasma membrane. Biochem. Biophys. Res. Comm. 239:42-45.

 

Stith, B.J., Goalstone, M.L., Espinoza, R., Smart T.(1997) sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis. Mol. Biol. Cell. 8(4):755-65.

 

Goalstone, M.L., and Draznin, B. (1998). What insulin does to cellular Ras. Cellular Signalling 10(5):297-301.

 

Goalstone, M.L., Leitner, J.W., Wall, K., Dolgonos, L., Rother, K.I., Accili, D., and Draznin, B. (1998). Insulin’s effect on farnesyltransferase: specificity of insulin action and potentiation of nuclear effects of insulin-like growth factor-1, epidermal growth factor, and platelet-derived growth factor. J. Biol. Chem. 273:23892-23896

 

Goalstone, M.L., Leitner, J.W., and Draznin, B. (1998) Mechanism of insulin’s ability to potentiate mitogenic effects of growth factors. Adv. Cell. Mol. Endo. 3:155-173.

 

Goalstone, M.L., Natarajan, R., Standley, P.R., Walsh, M.F., Leitner, J.W., Carel, K., Scott, S., Nadler, J., Sowers, J.R., and Draznin, B. (1998). Insulin potentiates platelet-derived growth factor action in vascular smooth muscle cells. Endocrinology 139: 4067-4072.

 

Marc L. Goalstone and Boris Draznin. (1999). Effect of insulin on farnesyltransferase gene transcription and mRNA stability. Biochem. Biophys. Res. Comm. 254:243-247.

 

Goalstone, M.L., Wall, K., Leitner, J.W., Kurowski, T., Ruderman, N., Pan, S.J., Ivy, J.L., Moller, D.E., and Draznin, B. (1999) Increased amounts of farnesylated p21Ras in tissues of hyperinsulinemic animals. Diabetologia 42:310-316.

 

Marc L. Goalstone, J. Wayne Leitner, Inga Golovchenko, M. Richard Stjernholm, Mireille Cormont, Yannick Le Marchand-Brustel, and Boris Draznin. (1999)  Insulin promotes phosphorylation activation of geranylgeranyltransferase II: Studies with geranylgeranylation of Rab-3 and Rab-4.  J. Biol. Chem. 274:2880-2884.

 

Boris Draznin, Philip D.G. Miles, Yolanta Kruszynska, Jerrold M. Olefsky, Jacob E. Friedman, Marc L. Goalstone, Inga Golovchenko, Richard Stjernholm, Kei Wall, and J. Wayne Leitner. (2000). Effects of insulin on prenylation as a mechanism of potentially detrimental influence of hyperinsulinemia. Endocrinology 141:1310-1316.

 

Goalstone ML, and Sadler SE (2000).  Analysis of Farnesyl Transferase activity during hormone induced maturation of Xenopus laevis oocytes. J. Exper. Biol. 286:193-203.

 

Chappell, J., Golovchenko, I., Wall, K., Stjernholm, R., Leitner, J.W., Goalstone, M.L., and Draznin, B. (2000).  Potentiation of Rho-A mediated LPA activity by hyperinsulinemia.         J. Biol Chem. 275:31792-31797

 

Golovchenko I, Goalstone ML, Watson P, Brownlee M, and Draznin B. (2000). Hyperinsulinemia enhances transcriptional activity of NFkB induced by angiotensin II, hyperglycemia and advanced glycosylation end products in vascular smooth muscle cells. Circ. Res. 87:746-752

 

Marc L. Goalstone, J. Wayne Leitner, Paulos Berhanu, Prem M. Sharma, Jerrold M. Olefsky, and Boris Draznin (2000) Insulin signals to prenyl transferases via the Shc branch of the intracellular signaling. J. Biol. Chem. 276:12805-12812.

 

Elizabeth Stephens, Patti J. Thureen, Marc L. Goalstone, Marianne S. Anderson, J. Wayne Leitner, William W. Hay, Jr., and Boris Draznin (2001) Fetal hyperinsulinemia increases farnesylation of p21Ras in fetal tissues. Am. J. Physiol. 281:E217-E223.

 

Dwight Klemm, J. Wayne Leitner, Peter Watson, Albina Nesterova, Jane E-B Reusch, Marc L. Goalstone, and Boris Draznin (2001).  Insulin–induced adipocyte differentiation: Activation of CREB rescues adipogenesis from the arrest caused by inhibition of prenylation. J. Biol. Chem. 276:28430-28435.

 

Curtis Scott Solomon and Marc Lee Goalstone (2001) Dominant Negative Farnesyltransferase a-subunit inhibits insulin mitogenic effects. Biochem. Biophys. Res. Comm. 285:161-166.

 

James Chappell, J. Wayne Leitner, Scott Solomon, Inga Golovchenko, Marc L. Goalstone, Boris Draznin (2001) Effect of insulin on cell cycle progression in MCF-7 breast cancer cells: direct and potentiating influence. J. Biol. Chem. 276:38023-38028.

 

Monica Montagnani, Inga Golovchenko, Injune Kim, Gou Young Koh, Marc L. Goalstone, Ameya N. Mundhekar, Mette Johansen, Dennis F. Kucik, Michael J. Quon, and Boris Draznin (2002).  Inhibition of phosphatidylinositol 3-kinase enhances mitogenic actions of insulin in endothelial cells.  J. Biol. Chem.  277:1794-1799.

 

Curtis Scott Solomon and Marc Lee Goalstone (2002) Dominant Negative a-Subunit of Farnesyltransferase Inhibits the Mitogenic Effects of Insulin and IGF-1 in MCF-7 Breast Cancer Cells. Biochem. Biophys. Res. Comm. 291:458-465.

 

Curtis Scott Solomon, J. Wayne Leitner, and Marc Lee Goalstone (2003) Dominant Negative a-Subunit of Farnesyl- and Geranylgeranyltransferase Inhibits Insulin-induced Differentiation of 3T3-L1 Pre-adipocytes.  J. Int’l. Obesity (27:40-47).

 

Inga Golovchenko, Chul-hak Yang, Marc L. Goalstone and Boris Draznin.  (2003) Garlic Extract Methylallyl Thiosulfinate Blocks Insulin Potentiation of PDGF-stimulated Migration of Vascular Smooth Muscle Cells.   Met. Clin. and Exp. 52: 254-259.

 

Christina A. Finlayson, James Chappell, J.Wayne Leitner, Marc L. Goalstone, Maureen Garrity, Samia Nawaz, Theodore P. Ciaraldi, and Boris Draznin. (2003) Enhanced Insulin Signaling via Shc in Human Breast Cancer. Met. Clin. and Exp. 52:1606-1611.

 

Marc L. Goalstone and Boris Draznin. (2004) Insulin and the Ras-dependent signaling pathway.  Diabetes Mellitus: A fundamental and clinical text (3rd edition) (LeRoith, Taylor and Olefsky. Eds.), Lippincott, Williams and Wilkins. Philadelphia, PA. Pp 421-429.

 

Cecilia C. Wang, Marc L. Goalstone Boris Draznin. (2004) Molecular mechanisms of insulin resistance that impact on cardiovascular biology. Diabetes 53:2735-2740.

 

Cornier, MA, Donahoo WT, Pereira R, Gurevich I, Westergren R, Enerback S, Eckel PJ, Goalstone ML, Hill J, Eckel RH, and Draznin B. (2005) Insulin sensitivity determines the effectiveness of dietary macronutrient composition on weight loss in obese women. Obesity Research 13(4):703-709.

 

Girish Sharma and Marc L. Goalstone. (2005) Dominant Negative FTase (DNFTa) inhibits ERK5, MEF2C and CREB activation in adipogenesis. Mol & Cell. Endoc. 245:93-104.

 

Girish Sharma and Marc L. Goalstone (2007) Regulation of ERK5 by insulin and angiotensin-II in vascular smooth muscle cells. Biochem. Biophys. Res. Commun.

354:1078-1083.

 

Rajakrishnan Veluthakal, Hitchintan Kaur, Marc L. Goalstone, Anjaneyulu Kowluru  (2007) Dominant negative alpha-subunit of farnesyl- and geranyl-transferase inhibits glucose-, but not KCl- stimulated insulin secretion in INS 832/13 cells. Diabetes 56:204-210.

 

Marc Goalstone, Vasudeva Kamath, Anjaneyulu Kowluru, (2010) Glucose activates prenyltransferases in pancreatic islet b-cells. Biochem. Biophysic, Res. Comm. 391:895-898.

 

Marc L. Goalstone, (2011) ERK5: Novel IKKa-Kinase in Rat Hippocampal Canadian J. Neuro. Sci. 38:639-648.

 

Daniel Mackesy and Marc L. Goalstone. (2011) Insulin Augments Tumor Necrosis Factor-alpha-stimulated increases in VCAM-1 in Vascular Endothelial Cells.  J. Inflammation 8:34

 

Rajakrishnan Veluthakal, Hitchentan Kaur, Marc L. Goalstone, Anjaneyulu Kowluru (2012) High glucose, palmitate and thapsigargin stimulate caspase degradation of alpha-subunit of FTase and GGTase-1 in pancreatic beta cells (manuscript in progress).

 

Daniel Mackesy and Marc L. Goalstone. (2012) Inhibition of PI3-kinase Pathway Increases Insulin and TNFa-stimulated VCAM-1 Expression in Vascular Endothelial Cells: A Model of Insulin resistance Linked to Vascular Inflammation (manuscript in progress).

 

Daniel Mackesy and Marc L. Goalstone. (2012) Insulin and TNFa Stimulate Different Isoforms of NFkB in their upregulation of VCAM-1 in Vascular Endothelial Cells. (manuscript in progress).

​Courses Taught 

7/06 – Pres.

Associate Professor of Medicine, Division of Endocrinology, Metabolism and Diabetes, Department of Medicine, University of Colorado Denver, School of Medicine, Anschutz Medical Campus, Aurora, Colorado. Instructor for Medical Fellows.

7/00 – 7 06

Assistant Professor of Medicine, Division of Endocrinology, Metabolism and Diabetes, School of Medicine, University of Colorado at Denver Health Sciences Center, Denver, Colorado. Instructor for Medical Fellows.

9/00 – Pres.

Adjunct Professor of Biology, Department of Integrative Biology, University of Colorado Denver, College of Liberal Arts and Sciences (Downtown Campus). Courses: Cell Biology (lecture and online formats), General Biology, Endocrinology, and Biology for Computer Scientists, Engineers and Mathematicians

2/10 – Pres.

Honors Program Faculty, University Honors and Leadership Program, University of Colorado Denver, Denver, Colorado. Faculty mentor for student research fellows.

8/00 – 12/00

Adjunct Professor of Biology, Department of Biology, Regis University, Denver, Colorado. Courses: Cell Biology.

1/99 – 12/00

Senior Instructor, Division of Endocrinology, Metabolism and Diabetes, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado. Instructor for Fellows.

7/99 – 10/00

Senior Instructor, Department of Biology, University of Colorado at Denver, Denver, Colorado. Courses: Cell Biology (Lecture and online formats)

1/99 – 12/99

Instructor, Department of Biology, University of Colorado at Denver, Denver, Colorado. Courses: Cell Biology (lecture and online formats), Advance Topics in Biology “Growth Factors, Hormones, Cytokines and Cell Response” (Graduate Seminar).

1/93 – 5/95

Graduate Teaching Assistant, Department of Biological Sciences, University of Denver, Denver, Colorado. Courses: General Biology and Physiology.

9/90 – 12/92

Graduate Teaching Assistant, Department of Biology, University of Colorado at Denver. Courses: General Biology. Laboratory coordinator and instructor for summer classes.

9/74 – 11/75

Graduate Teaching Assistant, Department of Zoology, Colorado State University, Fort Collins, Colorado. Courses: General Biology Laboratory.