Our lab varicella pathogenesis and latency and reactivation in an animal model. Studies include the role of cell-mediated immunity in the regulation of varicella latency and reactivation. A bacmid-based approach is used to generate mutant varicella virus to study immune evasion by varicella viruses. State-of-the-art technologies such as multiplex PCR analysis is also used for simultaneous detection of multiple human herpes viruses in biological specimens. Finally, the laboratory uses diagnostic tools to understand neurological complications of zoster including postherpetic neuralgia.