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University of Colorado Denver

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Protein Folding and Function: Dr. Mallela Research Laboratory

Publications


  1. S.M. Singh, S. Bandi, D.D. Shah, G. Armstrong, and K.M.G. Mallela, Missense mutation Lys18Asn in dystrophin that triggers X-linked dilated cardiomyopathy decreases protein stability, increases protein unfolding, and perturbs protein structure, but does not affect protein function, PLoS One, In Press (2014).
  2. R.L. Bis, S.M. Singh, J. Cabello-Villegas, and K.M.G. Mallela, Role of benzyl alcohol in the unfolding and aggregation of interferon alpha-2a, Journal of Pharmaceutical Sciences, In Press (2014). AbstractFull textPDF
  3. R.L. Bis and K.M.G. Mallela, Antimicrobial preservatives induce aggregation of interferon alpha-2a: The order in which preservatives induce protein aggregation is independent of the protein, International Journal of Pharmaceutics, 472 (2014) 356-361. AbstractFull textPDF
  4. R.L. Bis, T.M. Stauffer, S.M. Singh, T.B. Lavoie, and K.M.G. Mallela, High yield soluble bacterial expression and streamlined purification of recombinant human interferon alpha-2a, Protein Expression and Purification, 99 (2014) 138-146. AbstractFull textPDF
  5. S. Bandi, S.M. Singh, and K.M.G. Mallela, The C-terminal domain of the utrophin tandem calponin-homology domain appears to be thermodynamically and kinetically more stable than the full-length protein, Biochemistry, 53 (2014) 2209-2211. AbstractFull TextPDF
  6. S.M. Singh, S. Bandi, S.J. Winder, and K.M.G. Mallela, The actin binding affinity of the utrophin tandem calponin-homology domain is primarily determined by its N-terminal domain, Biochemistry, 53 (2014) 1801-1809. AbstractFull TextPDF
  7. R.L. Hutchings, S.M. Singh, J. Cabello-Villegas, and K.M.G. Mallela, Effect of antimicrobial preservatives on partial protein unfolding and aggregation, Journal of Pharmaceutical Sciences, 102 (2013) 365-376. AbstractFull textPDF
  8. S.M. Singh and K.M.G. Mallela, The N-terminal actin-binding tandem calponin-homology (CH) domain of dystrophin is in a closed conformation in solution and when bound to F-actin, Biophysical Journal, 103 (2012) 1970-1978. AbstractFull textPDF. Selected for a New & Notable: Biophysical Journal, 103 (2012) 1818-1819. Full textPDF
  9. S.M. Singh, J.F. Molas, N. Kongari, S. Bandi, G.S. Armstrong, S.J. Winder, and K.M.G. Mallela, Thermodynamic stability, unfolding kinetics, and aggregation of the N-terminal actin binding domains of utrophin and dystrophin, Proteins: Structure, Function, and Bioinformatics, 80 (2012) 1377-1392. AbstractFull textPDF
  10. S.M. Singh, R.L. Hutchings, and K.M.G. Mallela, Mechanisms of m-cresol induced protein aggregation studied using a model protein cytochrome c, Journal of Pharmaceutical Sciences, 100 (2011) 1679-1689. AbstractFull textPDF
  11. S.M. Singh, N. Kongari, J. Cabello-Villegas, and K.M.G. Mallela, Mutations in dystrophin that trigger muscular dystrophy decrease protein stability and lead to cross-beta aggregates, Proceedings of the National Academy of Sciences of the United States of America, 107 (2010) 15069-15074. AbstractFull textPDF
  12. S.M. Singh, J. Cabello-Villegas, R.L. Hutchings, and K.M.G. Mallela, Role of partial protein unfolding in alcohol-induced protein aggregation, Proteins: Structure, Function, and Bioinformatics, 78 (2010) 2625-2637. AbstractFull textPDF
  13. K.M.G. Mallela, Pharmaceutical Biotechnology – Concepts and Applications, Human Genomics, 4 (2010) 218-219. Full textPDF
  14. S. Bédard, M.M.G. Krishna, L. Mayne, and S.W. Englander, Protein folding: Independent unrelated pathways or predetermined pathway with optional errors, Proceedings of the National Academy of Sciences of the United States of America, 105 (2008) 7182-7187. (Equal contribution) AbstractFull textPDF
  15. S.W. Englander, L. Mayne, and M.M.G. Krishna, Protein folding and misfolding: Mechanism and principles, Quarterly Reviews of Biophysics, 40 (2007) 287-326. AbstractFull textPDF
  16. M.M.G. Krishna, H.Maity, J.N. Rumbley, and S.W. Englander, Branching in the sequential folding pathway of cytochrome c, Protein Science, 16 (2007) 1946-1956. AbstractFull textPDF
  17. M.M.G. Krishna and S.W. Englander, A unified mechanism for protein folding: Predetermined pathways with optional errors, Protein Science, 16 (2007) 449-464. AbstractFull textPDF 
  18. M.M.G. Krishna, H. Maity, J.N.Rumbley, Y. Lin, and S.W. Englander, Order of steps in the cytochrome c folding pathway: Evidence for a sequential stabilization mechanism, Journal of Molecular Biology, 359 ( 2006) 1410-1419. AbstractJournal coverFull textPDF
  19. R. Pidikiti, T. Zhang, K.M.G. Mallela, M. Shamim, K.S. Reddy, and J.S. Johansson, Sevoflurane-induced structural changes in a four-alpha-helix bundle protein, Biochemistry, 44 ( 2005) 12128-12135. AbstractFull textPDF
  20. R. Pidikiti, T. Zhang, K.M.G. Mallela, M. Shamim, K.S. Reddy, and J.S. Johansson, Structural changes in a four-alpha-helix bundle protein following sevoflurane binding, International Congress Series, 1283 ( 2005) 155-159. AbstractFull textPDF
  21. R. Pidikiti, M. Shamim, K.M.G. Mallela, K.S. Reddy, and J.S. Johansson, Expression and characterization of a four-alpha-helix bundle protein that binds the volatile general anesthetic halothane, Biomacromolecules, 6 ( 2005) 1516-1523. AbstractFull textPDF
  22. H. Maity, M. Maity, M.M.G. Krishna , L. Mayne, and S.W. Englander, Protein folding: The stepwise assembly of foldon units, Proceedings of the National Academy of Sciences of the United States of America, 102 (2005 ) 4741-4746. AbstractFull textPDF
  23. M.M.G. Krishna and S.W. Englander, The N-terminal to C-terminal motif in protein folding and function, Proceedings of the National Academy of Sciences of the United States of America, 102 ( 2005) 1053-1058. AbstractFull textPDF
  24. M.M.G. Krishna, Y. Lin, and S.W. Englander, Protein misfolding: Optional barriers, misfolded intermediates, and pathway heterogeneity, Journal of Molecular Biology, 343 ( 2004) 1095-1109. AbstractFull textPDF
  25. M.M.G. Krishna, L. Hoang, Y. Lin, and S.W. Englander, Hydrogen exchange methods to study protein folding, Methods, 34 ( 2004) 51-64. AbstractFull textPDF
  26. M.M.G. Krishna, Y. Lin, L. Mayne, and S.W. Englander, Intimate view of a kinetic protein folding intermediate: Residue-resolved structure, interactions, stability, folding and unfolding rates, homogeneity, Journal of Molecular Biology, 334 ( 2003) 501-513. AbstractFull textPDF
  27. L. Hoang, H. Maity, M.M.G. Krishna, Y. Lin, and S.W. Englander, Folding units govern the cytochrome c alkaline transition, Journal of Molecular Biology, 331 ( 2003) 37-43. AbstractFull textPDF
  28. M.M.G. Krishna, Y. Lin, J. Rumbley, and S.W. Englander, Cooperative omega loops in cytochrome c: Role in folding and function, Journal of Molecular Biology, 331 ( 2003) 29-36. AbstractFull textPDF
  29. L. Hoang, S. Bédard, M.M.G. Krishna, Y. Lin, and S.W. Englander, Cytochrome c folding pathway: Kinetic native-state hydrogen exchange, Proceedings of the National Academy of Sciences of the United States of America, 99 ( 2002) 12173-12178. AbstractFull textPDF
    Corrections: Proceedings of the National Academy of Sciences of the United States of America, 99 ( 2002) 15831. Full textPDF
  30. M.M.G. Krishna, Studying how a protein folds, The Alchemist (Web journal of chemweb.com), 2002. PDF
  31. S.W. Englander and M.M.G. Krishna, Hydrogen exchange, Nature Structural Biology, 8 ( 2001) 741-742. Full textPDF
  32. A.S.R. Koti, M.M.G. Krishna, and N. Periasamy, Time-resolved area-normalized emission spectroscopy (TRANES): A novel method for confirming emission from two excited states, Journal of Physical Chemistry A 105 ( 2001) 1767-1771. AbstractFull textPDF
  33. M.M.G. Krishna, A. Srivastava, and N. Periasamy, Rotational dynamics of surface probes in lipid vesicles, Biophysical Chemistry, 90 ( 2001) 123-133. AbstractFull textPDF
    Erratum: Biophysical Chemistry, 91 ( 2001) 209. Full textPDF
  34. A. Mishra, G.B. Behera, M.M.G. Krishna, and N. Periasamy, Time-resolved fluorescence studies of aminostyrylpyridinium dyes in organic solvents and surfactant micelles, Journal of Luminescence, 92 ( 2001) 175-188. AbstractFull textPDF
  35. M.M.G. Krishna, J. Samuel, and S. Sinha, Brownian motion on a sphere: Distribution of solid angles, Journal of Physics A: Mathematical and General, 33 ( 2000) 5965-5971. AbstractFull textPDF
    Also on ArXiv: http://arxiv.org/abs/cond-mat/0005345v3 PDF
  36. M.M.G. Krishna, R. Das, N. Periasamy, and R. Nityananda, Translational diffusion of fluorescent probes on a sphere: Monte Carlo simulations, theory and fluorescence anisotropy experiment, Journal of Chemical Physics, 112 ( 2000) 8502-8514. AbstractFull textPDF
  37. M.M.G. Krishna , Dynamics of Fluorescent Probes in Biological Systems, PhD Thesis, Tata Institute of Fundamental Research, University of Mumbai, India, 1999. PDF  © M.M.G. Krishna & N. Periasamy. Please cite the thesis when referring to unpublished results.
  38. M.M.G. Krishna and N. Periasamy, Location and orientation of DODCI in lipid bilayer membranes: Effects of lipid chain length and unsaturation, Biochimica et Biophysica Acta (Biomembranes), 1461 ( 1999) 58-68. AbstractFull textPDF
  39. M.M.G. Krishna, Excited state kinetics of the hydrophobic probe nile red in membranes and micelles, Journal of Physical Chemistry A, 103 ( 1999) 3589-3595. AbstractFull textPDF
    Additions and Corrections: Journal of Physical Chemistry A, 103 ( 1999) 4129. Full textPDF
    More Corrections: PDF
  40. M.M.G. Krishna and N. Periasamy, Orientational distribution of linear dye molecules in bilayer membranes, Chemical Physics Letters, 298 ( 1998) 359-367. AbstractFull textPDF
  41. M.M.G. Krishna, V.K. Rastogi, N. Periasamy, and K.V.R. Chary, Fluorescence and NMR studies on human seminal plasma prostatic inhibin: Association of lifetimes with sterically constrained tryptophans, Journal of Physical Chemistry B, 102 ( 1998) 5520-5528. AbstractFull textPDF
  42. M.M.G. Krishna and N. Periasamy, Fluorescence of organic dyes incorporated in lipid membranes: Site of solubilization and the effects of viscosity and refractive index on lifetimes, Journal of Fluorescence, 8 ( 1998) 81-91. AbstractFull textPDF
  43. N.C. Maiti, M.M.G. Krishna, P.J. Britto, and N. Periasamy, Fluorescence dynamics of dye probes in micelles, Journal of Physical Chemistry B, 101 ( 1997) 11051-11060. AbstractFull textPDF
  44. M.M.G. Krishna and N. Periasamy, Spectrally constrained global analysis of fluorescence decays in biomembrane systems, Analytical Biochemistry, 253 ( 1997) 1-7. AbstractFull textPDF

For comments, suggestions and complaints, please email Krishna.Mallela@ucdenver.edu.

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