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Metabolomics Assays Offered as of 12/27/18


Metabolomics is the study of the unique chemical fingerprints of cellular processes.  It provides a snapshot of what is happening within an organism at the time of sampling.  Untargeted Metabolomics is a “shotgun” approach that can detect 100’s to 1,000’s of endogenous and exogenous compounds in tissues, cells and biological fluids (Click here for more information).  This technique is used to compare relative differences between sample groups (e.g. Disease vs. Healthy Control, Treatment vs. Control, Treatment Time Points, etc.).  By not limiting the analysis to a specific subset of compounds, data is generated to test a hypothesis, while simultaneously providing information that may be lead to additional biological differences (Reisdorph Lab Citations)​.


Assays Offered:

 

·        Various Coverage of the Metabolome Depending on Sample Preparation (Click here for more information).

·        Methanol Protein Precipitation Extraction

  • Methanol Extraction is suitable for certain cellular extracts
  • Methanol extracts typically use one chromatographic method

·        Methyl tert-Butyl Ether (MTBE) Liquid-Liquid Extraction (LLE) (JOVE Publication)​.

  • Higher coverage can be obtained by separating the hydrophobic and hydrophilic compounds using LLE
  • Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
  • Suitable for cell, biofluid and tissue samples

·        “Medium Throughput” MTBE Liquid-Liquid Extraction

  • Similar coverage as our traditional MTBE Liquid-Liquid Extraction Method, with higher daily sample prep throughput
  • Hydrophobic and hydrophilic fractions are analyzed using suitable chromatographic methods
  • Suitable for cell, biofluid and tissue samples

·        Urine Samples

  • Urine samples do not generally need to undergo an “extraction,” but do require some sample handling
  • Urine Samples typically use one chromatographic method.

​We also offer sample preparation training if you or a member of your lab would like to learn the protocol(s) suitable to process your own samples. Please contact Katrina Doenges (katrina.doenges@ucdenver.edu) for additional information.