Operetta High-Content Imaging and Analysis System (Perkin Elmer)
The Operetta is a cutting-edge, second generation high content screening instrument. It can image live or fixed cells in plates with any well density. It is equipped with three objectives (10X High NA, 20X Long WD, 40X High NA), and excitation and emission filters for nearly any fluorescent label or combination of labels. Additional objectives and filters can be purchased from Perkin Elmer if a protocol requires them. Other features of the Operetta include:
- Confocal optics: On-the-fly switchable between standard fluorescence and confocal modes for greater visual clarity within the focal plane
- Brightfield (transmission) imaging
- Live-cell chamber (CO2 and temperature controlled)
- Sophisticated image analysis software: Harmony software for quantification of virtually any cellular, subcellular, or multicellular response.
Image Analysis Options: The Operetta instrument and Harmony software come with a number of pre-configured assays. Although adjustments need to made to any assay, these serve as excellent starting points for building any sort of customized analysis algorithm.
Pre-configured analysis methods include: |
Cell Counting |
Receptor Internalization |
Live / Dead Cell Counting |
DNA Content |
Nuclear Shrinking |
Nuclear Fragmentation |
Marker Quantification in Nucleus /
Cytoplasm / Membrane |
Cell Cycle Classification |
Spot Analysis |
Cell Shape – Cell Rounding |
Translocation: Cyto-nuclear,
Cyto-membrane |
Fluorescence Redistribution – Cytoskeleton |
Mitotic Index |
Phenotype Classification – Cytoskeleton |
Nuclear Spots |
Lipid Droplet Analysis |
Nuclei Counting |
Colony Formation |
Neurite Outgrowth Analysis |
Cell Migration
|
Texture Analysis |
Micronucleus Analysis |