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University of Colorado Denver

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Tom Anchordoquy, PhD

Professor, Department of Pharmaceutical Sciences


Mailing address:

University of Colorado School of Pharmacy
Mail Stop C238
12850 E. Montview Blvd. V20-4120
Aurora, CO 80045

Office Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
Fourth Floor
Room 4120

Lab Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
Fourth Floor
Room 4440A

Contact:

Affiliations:

  • Member, Center for Pharmaceutical Biotechnology
  • Member, University of Colorado Cancer Center

Training and Education:

  • BS, Oregon State University (Biology)
  • MA, PhD, University of California at Davis (Zoology)
  • Postdoctoral Fellow, University of Colorado at Boulder and University of Colorado Denver.

Research Interest:

My laboratory focuses on the development of synthetic delivery systems for use in nucleic acids-based therapies (e.g., gene, siRNA, antisense, aptamers).  Our most recent work utilizes an unconventional approach toward developing lipid-based delivery systems that possess prolonged circulation lifetimes and target tumors.  Studies on our delivery systems include biophysical characterization, pharmaceutical optimization, cell culture assessment, and ultimately testing in animal models. 

In addition, our research focuses on the stability of delivery systems during physical stresses, e.g., freezing and drying. The goal of this research is to develop formulations for therapeutic use that will be stable on a pharmaceutically-relevant timescale (~ 2 years).  While many of our previous studies have involved acute stresses, our current stability work focuses on prolonged storage of polynucleotides and delivery systems in the dried state. 

As part of this work, we are attempting to determine the factors responsible for structural and chemical degradation, and identify strategies for maintaining physico-chemical characteristics and biological activity during prolonged storage.  These projects are ultimately aimed at harnessing the enormous potential of nucleic acid-based therapeutics by developing better methods for delivery and formulation.  Our laboratory is also conducting traditional pharmaceutical research involving the formulation and delivery of small molecule therapeutics.  This latter research is translational in nature, and recent efforts focus on developing topical formulations to assist in the treatment of breast cancer and melanoma patients.

Teaching:

  • Professional Program: Pharmaceutics, Compounding
  • Graduate Program: Membrane Dynamics, Ethics

Representative Publications:

  • Betker JL, Gomez J, and Anchordoquy TJ.  The effects of lipoplex formulation variables on the protein corona and comparisons with in vitro transfection efficiency.  J. Cont. Rel. 171:261-268 (2013).
  • Payton NM, Wempe MF, Betker JL, Randolph TW, Anchordoquy TJ. Lyophilization of a triply unsaturated phospholipid: Effects of trace metal contaminants. Eur. J. Pharm. Biopharm. 85:306-313 (2013).
  • Betker JL, Kullberg M, Gomez J, Anchordoquy TJ.  Cholesterol domains enhance transfection.  Therapeutic Delivery 4(4):453-62 (2013).
  • Epple LM, Griffiths SG, Dechkovskaia AM, White J, Anchordoquy TJ, Bemis LT, Graner MW.  Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles PLoS ONE 7(7):e42064. Epub 2012 Jul 27 (2012).
  • Xu L, Betker J, Yin H, Anchordoquy TJ. Ligands located within a cholesterol domain enhance gene delivery to the target tissue.  J. Cont. Release 160:57-63 (2012)