Want to purify cell populations for downstream assays? Sorted cells can be used for genetic analyses, proteomics studies, protein expression (by western blot), cellular assays (cytokine secretion, proliferation, killing assays, drug treatment assays, developmental progression, differentiation with individual culture conditions, and more), or to cell sorting can separate different populations of genetically-modified cells (transfections, RNAi knockdown, CRISPR targeted cells). At HIMSR, we offer full service cell sorting
and can prepare your sample, stain with the antibody panel of interest, and sort specific cell populations. We can also run your pre-stained samples on our instruments. Log into iLabs
for service fees; contact Kim Jordan
for a quote.
Fluorescence-Activated Cell Sorting (FACs)
We can help you using the BD FACSAria Fusion
, a flow cytometry-based cell sorter capable of separating individual cells based on light scatter properties (forward/side scatter), membrane permeability (live/dead exclusion), and expression of up to 12 surface markers. With this instrument, you can separate up to 4 different cell populations at once, and drop individual cells into 96-well plates to expand clonal populations from individual cells. You can achieve a very high purity (>95%). We can help develop new antibody panels to analyze your cells of interest.
This technology is optimal for the separation of any sample in single cell suspension (peripheral blood, PBMCs, disaggregated tissue, cell lines). For most downstream assays, the cells will need to be sorted fresh (live), on the day of sample collection.
- 70um, 85um, and 100um nozzle tips
- 4 Lasers: 405, 488, 561, 640
- Biosafety cabinet for sorting human cells safely
- 4-way and plate sorting
Magnetic Bead Cell Enrichment
Cells can be enriched from heterogeneous populations using antibodies coupled to magnetic beads with the Miltenyi AutoMACS
system. This system allows both positive selection and negative “untouched” selection of cell populations. Compared to FACs sorting, you can typically start with a smaller cell number and achieve a higher cell yield in a shorter amount of time with a magnetic enrichment. Depending on the separation kit and markers used, this technique enriches for populations with typical purities near 90%. Please see the Miltenyi website for available reagent kits