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Redox Biology Unit


The Redox Biology Unit will provide consultation and offer technical support for the study of dysregulated redox regulating signaling in fibrotic diseases. This unit has established protocols for electron paramagnetic resonance (EPR) spectroscopy, a powerful methodology requiring specialized expertise.  EPR spectroscopy is the primary analytical tool in the field of redox signaling and free radical biology, as it is the only method that can measure transient free radical species directly. 
The Redox Biology Core will offer these state of the art measurements for in vitro models or biologic samples (eg: tissue, blood) utilizing novel EPR spin traps and probes to interrogate individual free radical species and cellular redox state in specific cellular compartments (eg: extracellular, cytosolic or mitochondrial). The Redox Biology Core will also provide consultation and protocols for other redox methodologies including antioxidant enzyme activites, redox-sensitive targeted expression vectors, or high-throughput assays for ROS production or markers of oxidative stress. These studies are valuable to the study of fibrosis and can complement other studies including metabolomics and mitochondrial metabolism (Seahorse and Oroboros).
Please contact Eva Grayck for more information. Eva.grayck@ucdenver.edu

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