As a member of the Center for NeuroScience, you have access to several core facilities located on the Anschutz Medical Campus. Please click on the links below for information regarding specific core services.
NINDS P30 Rocky Mountain Neurological Disorders Center Cores
Optogenetics encompasses a set of methods by which cellular function can be manipulated with light, allowing unprecedented spatiotemporal control of a rapidly expanding set of biological activities.
light microscopy or nanoscopy (i.e. microscopy with nanometer-scale resolution)
is defined as imaging with a resolution below the diffraction
limit of conventional light microscopy, which is ~200nm in the equatorial (xy)
and ~500 nm in the axial (z) dimension. Recently developed super-resolution methods
that allow light microscopy to image subcellular structure and molecular
localization on the 10s of nm scale have
revolutionized neurobiology by allowing neuroscientists to study the inner
workings of glia, axons,dendrites, and synapses in unprecedented detail that
had previously only been possible using electron microscopy (EM). In
particular, super-resolution fluorescence imaging has many advantages over EM including
much easier sample preparation and staining procedures as well as the ability
to be applied to not only
fixed, but also living cells and tissues. Several different super-resolution
fluorescence imaging methods have been developed,
such as STimulated-Emission Depletion (STED) microscopy and the related methods of
Stochastic Optical Reconstruction Microscopy (STORM) and Photo-activation
Localization Microscopy (PALM). RMNDC Nanoscopy Core B provides access to
instrumentation and technical support for NINDS-funded and other
investigators at the University of Colorado-Anschutz Medical Campus to
perform state-of-the-art STED and STORM/PALM super-resolution imaging, as well
as complementary fluorescence lifetime imaging
(FLIM)-based Forster-resonance energy transfer (FRET) imaging.
want to use Core B for STED, STORM/PALM, or FLIM-FRET imaging
should contact Dominik Stich, PhD (DOMINIK.STICH@UCDENVER.EDU).
The Behavior and In Vivo Neurophysiology core (BINC)
provides animal housing in close proximity to testing with identical light/dark
cycles. Standard behavioral assays for mice and rats (including
cognition, altered emotionality, motor function, sensory tasks and task
development) and continuous rodent behavioral (video) and
neurophysiological/EEG monitoring services (including cerebral electrical
patterns during waking and sleep) are provided to researchers.