The Program in Translational Lung Research seeks to provide state of the art experimental tools available to the division investigators. This program is made up of three cores:
Small Animal Modeling Core:
The core includes several of the tools needed to characterize mouse lung phenotypes, including pulmonary function testing using the Flexi-vent system, pulmonary arterial pressures and heart function, exercise testing, and real time lung imaging, among others.
The core processes tissues for subsequent paraffin embedding and histological sectioning; develops experimental immunohistochemical stains targeted at several functional and structural molecules; performs immunofluorescence against multiple markers; plans and implements stereological approaches to the quantification of anatomic and microscopic structures; performs laser capture microdissection for molecular and expression studies.
Research Design and Statistics
The core will provide means to plan experiments, including statistical support, notably of power analyses, overall design of experimental studies, and consideration of appropriateness of statistical testing.