Various types of biologic samples will be obtained from subjects enrolled in consortium investigations. Please see table below for complete details.
a. From subjects with alcohol use disorders and healthy controls. Enrolled individuals will have sampling conducted in a clinical translational research center (CTRC) or similarly controlled setting. Bronchoscopy with bronchoalveolar lavage (BAL) and brushings will be performed by the principal investigator or her co-investigators, all of whom are pulmonologists.
b. From burn subjects (patients). Enrolled patients will have data collection and sampling performed in the burn ICU setting, including validated alcohol questionnaires. Information regarding alcohol history is valid when collected by surrogate, and will be confirmed from patients that recover. Data collection, as well as biologic sampling will be performed by trained personnel. Bronchoscopy will be performed by the PI or her co-investigators, all of whom have extensive experience in research bronchoscopy.
Table. Description of subject type and planned consortium sample collection after screening and informed consent process.
| |
Single bronchoscopy protocol, uninjured volunteers |
Longitudinal bronchoscopy protocol (planned) |
Burn patient protocol |
|---|
| Subject type(s) |
AUD Subjects &
Pair-matched controls |
AUD subjects |
Burn ICU patients, with and without AUDs |
|---|
| Demographic and alcohol data |
Yes, from subject |
Yes, from subject |
Yes, from health care proxy (patient when possible) |
|---|
| Length of stay in CTRC/ICU |
1 (controls) to 3 (AUDs) days |
9 days |
varies |
|---|
| Blood (plasma, serum, buffy coat) |
On Day 1 |
Daily over 7 days |
On Day 1 |
|---|
| Bronchoscopic samples |
BAL, brushings (microbiologic & cytologic) on Day 1 |
BAL, brushings (microbiologic & cytologic) on Days 1 & 7 |
BAL on Day 1 |
|---|
| Exhaled breath condensate (EBC) |
On Day 1 |
On Days 1 & 7 |
On Day 1, 3, and 5 |
|---|
| Other |
Oropharyngeal sampling on day 1
Clean catch Urine on Day 1 |
Clean catch Urine on Day 1 and 7 |
Clean catch urine on day 1 |
|---|