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Genomic and Microarray Core Data Analysis


RNA-seq
The core offers a new fixed-price service for gene level Differential Expression (DE) analysis. The package includes sample quality assessment (QA), align sequencing reads to a target transcriptome and /or genome, and perform a detailed DE analysis. Customer will receive the following at the end of the analysis cycle:
  1. A QA report for the sample quality, mapping statistics and the vigor of the experiment in general; including the description of the method section for publication purposes (link to see sample QA report)
  2. Analysis Result Table of statistically significant up- and down-regulated genes; including all the raw count values, fold-change, p-value, FDR and fully annotated document in Excel format (link to download a sample analysis result table)
  3. Instruction to import mapped data (SAM/BAM files) onto Broad Institute Integrated Genome Viewer (IGV) to inspect read distribution  


data.png

(IGV example figure)

Future Service
We anticipate building similar packages for other routine NGS analysis
  1.  ChIP-seq
  2. Exome-seq