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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center
 

Flow Cytometry

Using flow cytometric analysis, high-speed cell sorting, microscopy and multiplexed fluorescent microsphere assays, the Flow Cytometry Shared Resource (FCSR) can perform a wide range of assays supporting cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis and cell phenotyping. These assays are crucial to understand how cancer derails normal cellular processes to cause disease.

FCSR has two sites, one at the University of Colorado Anschutz Medical Campus and one at National Jewish Health. We provide a consultation to help those CU Cancer Center members with little flow cytometry or immunology background to design appropriate experiments.

Services:

Flow Cytometry Analysis

We have four main analyzers, each best for different types of analysis. You may use the machines yourself or ask us to analyze your samples for you.

Beckman Coulter FC500
  • 2 lasers: 488 and 635
  • 5 color analysis
  • Suggested fluors: FITC, PE, PE-Texas Red, PE-Cy5 or APC, PE-Cy7 or APC-Cy7
Beckman Coulter Gallios & Gallios561
  • 3 & 4 lasers: 405, 488, 635, and 561 (Gallios 561 only)
  • Analysis rate of 25,000 events/second
  • 10 color analysis
  • Suggested fluors: FITC, PE, PE-TexasRed, PECy5.5, PECy7, APC, Alexa700, APC-Cy7, Pacific Blue, Pacific Orange. Gallios561: tdTomato, RFP, mCherry, Crimson
Beckman Coulter Cyan
  • 3 lasers: 488, 642 and 405
  • Analysis rate of 50,000 events/second
  • 100 million event data files
  • 9-color analysis
  • Suggested fluors: FITC, PE, PE-TexasRed, PECy5.5, PECy7, APC, APC-Cy7, Pacific Blue, Pacific Orange

Cell Sorting Services

We have two cell sorting machines with different capabilities. Core staff performs all cell sorts.

Moflo XDP 100
  • 100 µm nozzle tip for larger cells
  • 4 lasers: 405, 488, 561, 635
  • 10 color capability
  • Plate sorting for any size plate
  • 4-way sorting
Moflo XDP 70
  • 70 µm nozzle tip for smaller cells
  • 5 lasers: UV, 405, 488,552, 635
  • 14 color capability
  • Plate sorting for any size plate
  • 4-way sorting

Luminex System

This analysis measures cytokines, chemokines, phosphoproteins and apoptosis markers.

Vicell and Cytospin

  • ViCell Cell Counter-automated Trypan Blue cell counts
  • Cytospin microscope slide centrifuge