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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center
 

Flow Cytometry


Using flow cytometric analysis, high-speed cell sorting,  and multiplexed fluorescent microsphere assays, the Flow Cytometry Shared Resource (FCSR) can perform a wide range of assays supporting cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis and cell phenotyping. These assays are crucial to understand how cancer derails normal cellular processes to cause disease.

FCSR has two sites, one at the University of Colorado Anschutz Medical Campus and one at National Jewish Health. We provide a consultation to help those CU Cancer Center members with little flow cytometry or immunology background to design appropriate experiments.

Services:

Flow Cytometry Analysis

We have four main analyzers, each best for different types of analysis. You may use the machines yourself or ask us to analyze your samples for you.

Beckman Coulter FC500
  • 1 lasers: 488
  • 5 color analysis
  • Suggested fluors: FITC, PE, PE-Texas Red, PE-Cy5, PE-Cy7
Beckman Coulter Gallios
  • 3 lasers: 405, 488, 638
  • Analysis rate of 25,000 events/second
  • 10 color analysis
  • Suggested fluors: FITC, PE, PE-TexasRed, PECy5.5, PECy7, APC, Alexa700, APC-Cy7, Pacific Blue, BV510

Beckman Coulter Gallios  561
  • 4 lasers: 405, 488, 638, and 561
  • Analysis rate of 25,000 events/second
  • 10 color analysis
  • Suggested fluors: FITC, PE, PE-TexasRed, PECy5.5, PECy7, APC, Alexa700, APC-Cy7, Pacific Blue, BV510.
  • Hypercyt 96-well plate reader


Beckman Coulter Cyan
  • 3 lasers: 488, 642 and 405
  • Analysis rate of 50,000 events/second
  • 100 million event data files
  • 9-color analysis
  • Suggested fluors: FITC, PE, PE-TexasRed, PECy5.5, PECy7, APC, APC-Cy7, Pacific Blue, BV510

Cell Sorting Services

We have three MoFlo high-speed cell sorting instruments with different capabilities. All three sorters can sort into any size tube or plates.

Moflo XDP 100
  • 100 µm nozzle tip for larger cells
  • 4 lasers: 405, 488, 561, 641
  • 4-way sorting
Moflo XDP 70
  • 70 µm nozzle tip for smaller cells
  • 5 lasers: UV, 405, 488,552, 635
  • 4-way sorting

Moflo Astrios EQ
  • Enhanced forward scatter for small particle detection
  • Biosafety cabinet for sorting human samples
  • 5 lasers: 405, 488, 532, 561, 640
  • 6-way sorting


Luminex Magpix System

This analysis measures cytokines, chemokines, phosphoproteins and apoptosis markers.

Vicell and Cytospin

  • ViCell Cell Counter-automated Trypan Blue cell counts
  • Cytospin microscope slide centrifuge