Services Provided by the Core
The Core will conduct pronuclear
injection of DNA constructs (e.g. conventional
transgenics, BAC transgenics). Our goal is to implant 150 embryos into recipient mice. On average, we
expect 20 pups to be born from each experiment.
If less than 5 pups are born, we will repeat the
injection without charging labor costs (the
will have to pay animal costs and supplies). Due to the nature of the experiment,
Core cannot guarantee that transgene-expressing animals are born.
of the Investigator
The investigator will provide the
DNA construct. Please inquire regarding preferred DNA preparation
protocols and required quality control experiments to be performed
by the investigator. Please note that Core staff can also purify and prepare high quality DNA for an additional charge.
The investigator is responsible
for obtaining regulatory approval to generate transgenic mice (IBC,
IACUC). The Core has generated standardized production protocols to be used by investigators.
Contact us for more information.
International Society for Transgenic