The Bioengineering Core, which is supported by the Gates Center for Regenerative Medicine, the Dean of the Medical School, and the Department of Dermatology, is dedicated to assist you in developing stem cell-based and animal-based research models. Our animal component provides all services needed to generate genetically engineered (e.g. transgenic) mouse models. We advise on gene targeting vector design, conduct gene targeting experiments in mouse embryonic stem (ES) cells (e.g. knockout and knockin mice) and conduct all embryo manipulations required to generate mouse models (e.g generation of ES-based chimeric mice, conventional transgenic mice, CRISPR/CAS-mediated genome editing in mouse embryos). We also preserve valuable mouse models by sperm cryo-preservation and embryo cryo-preservation. These services ensure that you will not lose important mouse models to events such as disease outbreaks and gene drift.
The Bioengineering Core also generates custom-designed induced Pluripotent Stem Cells (iPSC) from various species (e.g. human, mouse, dog, horse) using reprogramming methods that do not leave a genetic footprint in these cells. iPSC have biological properties similar to embryonic stem (ES) cells. These cells can be generated from adult tissue cells (e.g. skin fibroblasts) by the introduction of a combination of transcription factors (reprogramming factors). iPSC can be differentiated into many cell lineages.
Consequently, it is anticipated that iPSC will become essential tools for the generation of replacement tissues in regenerative medicine, for the screening of new drugs and small molecules aimed at modifying specific cellular processes, and for the design of new therapeutic strategies to treat genetic diseases. Besides iPSC generation and characterization, we assist investigators in developing protocols to differentiate iPSC into defined somatic cell lineages. Lastly, we are offering gene-editing of iPSC and mouse embryos using CRISPR/CAS technology.
Peter J Koch, PhD
Professor of Dermatology, Cell & Developmental Biology
Peter J. Koch, PhD
Professor of Dermatology, Cell & Developmental Biology and Ophthalmology
Director, Bioengineering Core
Saiphone Webb, BS
Senior PRA, Core Manager
Embryo Manipulation Services, Stem Cell Generation and Gene Targeting in Stem Cells
Jiangli Chen, DVM, PhD
Genome Editing in Stem Cells and Mouse Embryos
Kenneth Barentsen, BS
Embryo Manipulation Services
Wallace Chick, PhD
Gene Targeting in ES Cells and Vector Development