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Reprogramming


​​The core uses a patent-pending, modified mRNA/miRNA-based method to reprogram somatic cells into induced pluripotent stem cells (iPSCs). This cutting-edge technology was developed by the co-directors of the core, Drs. Kogut and Bilousova (High-efficiency RNA-based reprogramming of human primary fibroblasts. Nature Communications. (2018). https://doi.org/10.1038/s41467-018-03190-3). It provides the following significant advantages over traditional cell reprogramming approaches such as lentiviral-, episomal-, and Sendai virus-based methods:
  • non-integrative
  • ultra-high yield (up to 90%)
  • cost effective
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We provide up to 5 early passage iPSC clones per reprogrammed cell line (2 vials per clone)

Fibroblast reprogramming

Primary fibroblast lines provided by a customer, or isolated by the core from provided skin biopsies, are reprogrammed into iPSCs. Our protocol is approximately 18 days (iPSC colonies can be seen as early as day 13). If desired, a complete characterization profile can be provided post reprogramming.

​Service Time​
​Fibroblast Isolation From Skin Biopsy ​2 weeks
​Fibroblast Reprogramming ​2-3 weeks
​iPSCs culturing and expansion ​2 weeks
​Mycoplasma test ​1 week
​Pluripotency Staining ​1 week
​Karyotyping​ ​4 weeks


Renal cell reprogramming

The core personnel is skilled in developing primary renal epithelial cell (REC) lines from human urine specimens and reprogramming REC lines into iPSCs. Please contact us​ for more information.

Note: a copy of approved IRB protocol(s) must be provided by customers before reprogramming patient-derived cells

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