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Modified mRNA

​​In vitro-transcribed mRNA offers several distinct advantages for ectopic gene expression compared to more common plasmid- or viral-based expression systems:
  • Non-integrating - Eliminates the possibility of insertional mutagenesis
  • Efficient Delivery – mRNA only needs to cross a single plasma membrane to enter the cytoplasm, where translation occurs. mRNA can have significantly higher transfection efficiencies than plasmids in some cell lines
  • Controlled expression – Dosage can be finely tuned by simply transfecting more or less mRNA
  • Rapid expression – Functional protein is translated quicker since transcription/polyadenylation/nuclear export does not need to occur
  • Transient expression – Plasmid- and viral-based expression can be detrimentally stable, or even permanent if random DNA integration occurs

Features of our modified mRNA:

  • 100% modified using 5-methyl-cytosine (5mC) and pseudouridine (ψ) to better mimic endogenous mRNA and reduce innate-immunity stimulation
  • Poly(A) tail introduced via transcription template so all Poly(A) tails are identical in length
  • Capped using anti-reverse cap analog (ARCA) for increased mRNA translation efficiency
  • DNase treated to remove DNA transcription template
  • Phosphatase treated to further reduce innate immune response
  • 5’ UTR includes Kozak consensus sequence for optimal translation efficiency
  • 3’ UTR derived from human β-globin gene, shown to increase mRNA stability
  • Highly-competitive price
  • Bulk discounts are available (up to 30% off)

The SCB&DM core has a growing library of mRNAs for purchase. We can also provide custom cloning and synthesis services for your gene of interest. Contact us​​ to inquire about custom orders to suit your experimental needs. ​

Reprogramming mRNAs
​mRNA ​Notes
Pre-Mixed Reprogramming mRNAs  3:1:1:1:1:1 mix of
M3O​ : SOX2 : KLF4 : cMYC : LIN28A : NANOG
M3O​​​​​ Human OCT4 fused to MyoD transactivation domain
SOX2 Human
KLF4 Human
cMYC​ Human​
LIN28A Human
NANOG​ Human
OCT4 Human

Cas9 variants
​mRNA ​Notes
​​SpCas9 wild-type Cas9​​
eSpCas9(1.1) High Fidelity Cas9
HypaCas9 High Fidelity Cas9
SpCas9n Cas9 nickase
SpCas9-hGEM Cas9 fused to Geminin for enhanced HDR
dCas9-VPR Dead SpCas9 fused to transcriptional activator

Other useful cDNAs
​mRNA ​Notes​
PuroR ​​Puromycin Resistance​​
Cre Cre Recombinase
BlastR​ Blasticidin Resistance
Fluorescent proteins
​​ ​mRNA ​Excitati​on ​​​​(nm) ​Emmision ​(nm) ​Estimated ​Brightness ​ ​Tags ​​ ​Notes
​399 ​454 ​32.4
​mTagBFP2 ​399 ​454 ​32.4 ​3xFlag, nls ​localized to nucleus
​T-Sapphire ​399​ ​511 ​26.4 ​Long stokes shift
​mCerulean3 ​433 ​475 ​32
​d2EGFP ​488 ​507 ​33.6 ​Destabilized EGFP
​mWasabi ​493 ​509 ​56
​mWasabi​ ​493 ​509 56 ​3xFlag, nls​​ localized to nucleus
​Clover ​505 ​515 ​84
​Clover-P2A- ​PuroR ​505 ​ ​515 ​ ​ ​ ​ ​ ​Bicistronic expression of Clover and Puromycin resistance ​
​nls-Clover ​505 ​515 ​84 ​3xFlag, nls ​localized to nucleus
​YPET ​517 ​530 ​80
​tdTomato ​554 ​581 ​95.2 ​3xFlag, nls localized to nucleus
​tdTomato-P2A- ​Blast ​554 ​ ​581 ​ ​ ​ ​Bicistronic expression of tdTomato and Blasticidin resistance
​nls-tdTomato ​554 ​581 ​95.2 ​3xFlag, nls ​localized to nucleus
​mRuby2 ​559 ​600 ​43
​mRuby2-P2A ​Blast ​559 ​ ​600 ​ ​ ​ ​ ​ ​Bicistronic expression of mRuby2 and Blasticidin resistance
​iRFP670 ​643 ​670 ​12.5
​​iRFP670 ​643 ​670 ​12.5 ​​3xFlag, CAAX ​​localized to membrane​​

*Brightness = extinction coefficient x quantum yield.
For reference, EGFP brightness = ~34.