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Gene Editing

The core utilizes the CRISPR/Cas9 system to achieve gene editing in iPSCs and somatic cells.
​Service ​Time
​​Experimental Design, Vector Assembly, Validation
​​Targeting strategy and design ​1 week
​Vector Construction ​1-4 weeks
​gRNA synthesis ​1 week
​Transfection into target cells ​1-2 weeks
​Genome editing verification ​1-2 weeks
Transfection into cells, INDEL confirmation
​Transfection into target cells ​1-2 weeks​
​Genome editing verification​ ​1-2 weeks
Single-cell expansion, genome editing confirmation
​Picking/expanding clones ​2-4 weeks
​Colony screening (T7E1, ddPCR, etc.) ​2-3 weeks
​Sanger Sequencing ​2-3 weeks
iPSC clone characterization, expansion, biobanking
​Cell recovery, culturing, expansion ​2-4 weeks
​Sequencing Targeted Region ​1-2 weeks
​Pluripotency Staining ​1 week
​Karyotyping ​2 weeks
​Mycoplasma ​1 week
​Freezing ​1 week
Total ​(20-35 weeks)​