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Molecular Biology Service Center

DNA Sample Submission Guidelines


DNA Sequencing:

To submit samples to the MBCF, first complete a sequencing request.

  • ​​Standard Reaction/Data Collection: samples should be submitted in the following form:
    1. For plasmid DNA <12kb - provide 20 µL of DNA at a concentration of 40 ng/µL in water.  For PCR products 300-600bp - provide 20 µL of DNA at a concentration of 10 ng/µL in water.
    2. For a custom primer, please provide 10 µL at a 10 µM concentration and include the Tm of the primer.
    3. For template/primer mix please provide a 20ul volume of template at 40ng/ul  and primer at 1uM final concentration
    4. For PCR fragment DNA- amplicons must be purified/treated prior to DNA sequencing. Please inquire for details and/or protocols. If you would like to submit PCR reaction, we can treat/prepare your sample for sequencing by purification or ExoSap treatment. Please inquire about cost and sample submission.
    5. The following standard primers are provided: M13F(-20), M13R,T7, T7 Term, SP6, T3, CMV-F, PGEX-F, PGEX-R, BGH-R, Lucif Ctrm Rev, GFP-R, pNTR1A-F, pNTR1A-R, PQE30-F, PQE-Tri-F, PQE-Tri-R, E-GFP-R, and V5 Ctrm Rev.
  • DNA samples that are submitted for standard ‘reaction/data collection’ service must be accurately quantified and prepared. Samples submitted with inaccurate concentrations and/or with contaminants present will result in poor sequence results or a failed reaction, which will still be billed to the user.
  • After processing the results will be available to you from the webpage. Please login and click on “View your sequencing results”.
  • The sequence will be repeated at no extra cost if there is an operator error or machine malfunction. Please provide accurately quantified, high quality DNA for successful results.
  • Automated sequencing is very sensitive to template and primer preparation.
  • It is essential that the templates be free from ethanol, salts and other contaminants, as these greatly affect the quality of the sequencing results.

NOTE: WE WILL NOT REPEAT SAMPLES AT NO CHARGE IF THE DNA IS OF BAD QUALITY OR INACCURATE CONCENTRATION.

Here are a couple programs available to view chromatogram files (.ab1):

Chromas Lite – from Technelysium
http://technelysium.com.au/?page_id=13

Finch TV – free download from Geospiza
http://www.geospiza.com/Products/finchtv.shtml

Chromatogram Explorer – from DNAbaser
http://www.dnabaser.com/download/chromatogram-explorer/

Human Identity Testing, Cell Line Authentication, and Mycoplasma Testing:

  • Please provide isolated/purified genomic DNA in a minimum volume of 15ul and a minimum concentration of 30ng/ul.
  • For ID and CLA testing only- a cell pellet can be submitted and we can perform the isolation for you. Standard DNA isolation rates apply.

Location: 
Barbara Davis Center
Anschutz Medical Campus
1775 Aurora Court, 4th floor, Room 4207
Phone: 303-724-6825

Molecular Biology contact personnel:
Tamara Max​Core Manager

The purpose of this website is to provide some basic information about the BDC Service Cores including contact information and links to on-line services where they are available. If you have questions about the BDC Service Cores please contact us (email or phone: 303-724-6836).