The objective of the BDC Molecular Biology Core Laboratory is to facilitate and promote the application of contemporary molecular biological techniques to existing endocrinology and diabetes research programs at University of Colorado Denver and its affiliated institutions. Diabetes researchers in our Research Base come from diverse clinical and scientific backgrounds including immunology, epidemiology, genetics, surgery, developmental biology, biochemistry, cellular biology, and molecular biology. The Core provides researchers with up-to-date and innovative molecular biology techniques which include:
- Automated DNA sequencing
- Automated fragment analysis (microsatellite, AFLP, extension PCR, MLPA and SNP analysis, Zygosity testing, cell line authentication)
- Quantitative Real-time PCR analysis including: gene expression, SNP genotyping, and High Resolution Melt analysis
- Amplicon Library Construction for NGS applications
- Speed Congenics
- High through-put SNP Genotyping
- Basic and Custom molecular services including protocol development and optimization, nucleic acid purification, and RNA/DNA isolation
In addition to providing molecular services, the Core provides hands-on training of many molecular biology techniques where researchers and their staff can gain basic skills working on projects specifically related to their interests. Consultation services are also provided for advanced training and collaboration. Please contact Mark Farrell, (Core Manager) for inquiries regarding your project.
Barbara Davis Center
University of Colorado, Anschutz Medical Campus
1775 Aurora Court, 4th floor, Room 4207
Aurora, CO 80045
Molecular Biology contact personnel:
Mark Farrell, Core Manager
The purpose of this website is to provide some basic information about the BDC Service Cores including contact information and links to on-line services where they are available. If you have questions about the BDC Service Cores please contact us (email or phone: 303-724-6836).