Islet Preparation Core
The Islet Core primarily harvests functional pancreatic islets from mice. Other organs of interest (spleen, pancreatic lymph nodes, thymus) may be collected at the same time if desired.
While services are requested through iLabs (Barbara Davis Center Bioresource Core), actual scheduling must be done by personally contacting the Core (phone or email).
Donor mice (client provides) are humanely euthanized, the in situ pancreas is injected with a collagenase/neutral protease cocktail, and the enzyme-inflated pancreas is removed. After incubation at 37°C the islets are denity-gradient purified from the digested pancreas, and ultimately the clean islets are hand-picked under the microscope.
The client may request the end product to be reported as either a simple islet count or scored as islet equivalents (IEQs; the islets are “scored” by two-dimensional size).
Harvested islets are scored as IEQs and transplanted under the kidney capsule of anaesthetized recipient(s). Recipient mice are also provided by the client; arrangements may be made to house transplanted animals in the BDC Animal Facility.
This transplantation service is also available for other tissue types (client provides).
Animal Protocol Requirement
Researchers requesting pancreatic islets from the Core must first possess a current IACUC-approved animal protocol. A protocol amendment is generally required; I need to be added to your protocol and you will need to include a section on our islet harvest / transplantation procedure(s). I will provide you with my PQF and the appropriate protocol narrative(s).
Human Islet Program
The BDC has a Master Material Transfer Agreement (MTA) in place with the Alberta Diabetes Institute. By providing a copy of their Institutional Biosafety Committee (IBC) approval, a brief description of their work, and signing on to the MTA, interested researchers have access to human islets at a cost of $0.10 CAD / IEQ (plus shipping). Islets are typically offered every week or two. Orders are placed through the Core and are delivered here for distribution.
Islet contact personnel:
Scott Beard, BDC Islet Core Manager