The Barbara Davis Center Cytometry Core has a new Cytek Aurora capable of acquiring up to 20 colorswith just three lasers (405nm Violet, 488nm Blue, and 640nm Red). A fourth laser (561nm Yellow/Green) will be added soon.
The Aurora employs 40 detector channels (blue laser FSC, violet laser SSC, 38 fluorescence) to generate full emission spectra signatures across all lasers. The use of spectral unmixing technology gives the user tremendous flexibility in fluorochrome selection. It is possible to use dyes with highly overlapping emission profiles (BV421 and Pacific Blue, for example) in the same sample and there are NO filters to change.
The Cytometry Core is a trained user-run facility, permitting researchers to have their own staff run samples. The Core manager will train your personnel to properly operate the Aurora.
Expertise (the Core manager and Cytek personnel) is available to help with cytometry-related concerns, including experimental layout, fluorochrome selection, sample preparation, collection, and analysis.
If desired, the Core Manager is available to assist you with sample acquisition and analysis.
The cytometer is maintained at the highest standard through the manufacturer’s (Cytek) preventative maintenance programs and by daily in-house Quality Control procedures.
Cytometry contact personnel:
Scott Beard, BDC Cytometer Core Manager