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Nichole Reisdorph, PhD

Associate Professor, Department of Pharmaceutical Sciences, and Director, Skaggs School of Pharmacy Mass Spectrometry Facility


Mailing address:

University of Colorado School of Pharmacy
Mail Stop C238
12850 E. Montview Blvd. V20-2120
Aurora, CO 80045​

Office Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
Second Floor
Room 2120

Lab Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
Second Floor
Room 2460D

Contact:

Training and Education

B.S. - Northern State University; Aberdeen, SD (Biology)
M.S. - University of South Dakota; Vermillion, SD (Biology)
Ph.D. - University of South Dakota; Vermillion, SD (Biochemistry and Molecular Biology)
Postdoctoral Fellow - The Scripps Research Institute, La Jolla, CA (Mass Spectrometry)

Research Interests

Asthma biomarkers (predictive/personalized medicine)

We are one of only a few groups that are utilizing small molecules to predict response to medication. We have discovered that urinary leukotriene E4 (uLTE4) is a potential biomarker for both asthma severity and response to LTRA therapy. Our recent work has expanded on this to include mechanistic studies of response to medication. Our metabolomics research is focusing on discovering biomarkers for response to medication, identifying novel pathways and drug targets, and determining how metabolites from the host and microbiome contribute to asthma pathogenesis and exacerbation.

Systems biology approaches to understanding Chronic Obstructive Pulmonary Disease (COPD)

COPD is a complex disease with multiple phenotypes. This research applies metabolomics and genomics to samples from a large human cohort (COPDgene) and an animal model to discover a role for sphingolipids in COPD. Validation is performed using independent samples and mass spectrometry to elucidate the role of specific species in various COPD phenotypes. For example, we discovered that sphingomyelins are positively correlated with emphysema; trihexosylceramides are negatively associated with exacerbations. To our knowledge, this was the first study to apply systems biology to COPD in both mice and humans. Subsequent experiments are focusing on additional pathways of interest. This work has also resulted in the development of an airway fluid small molecule database that can be used to support a variety of lung research.

Mass Spectrometry Method Development

Metabolomics and proteomics are relatively new fields of science, especially when applied to clinical research. Our laboratory has been responsible for developing several methods that are being applied to clinical metabolomics and proteomics; these include methods of profiling over 4,000 small molecules in plasma, developing quantitative methods for leukotrienes and amino acids, and applying novel proteomics methods to quantitate phosphorylation sites on cardiac proteins.

Autoantigens of Type I Diabetes (T1D)

This collaborative work resulted in the discovery of novel autoantigens for T1D; historically, insulin has been thought to be the primary autoantigen. We utilized a novel proteomics workflow to determine that Chromogranin A and IAPP are two additional autoantigens in T1D. This has since resulted in the discovery of novel modifications that confer antigenicity to a number of granular proteins.

Training Courses

Hands-On Training workshops in Omics

Hands-on training in the fields of proteomics, metabolomics, and informatics is often difficult to access; this is in part due to the lack of organized courses that provide access to instrumentation and software. Our lab has developed innovative curricula for several hands on workshops that have reached over 400 international participants since 2004, including 4-day proteomics, 3-day metabolomics, 3-day clinical proteomics, 1-day bioinformatics, and 5-day proteomic and metabolomic hands on workshops. A total of 38 workshops have been held to date, including an NHLBI-funded genomics and proteomics workshop series. 4-day Metabolomics and proteomics workshops are offered annually through our lab.

Publications​​​