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University of Colorado Denver

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Neera Tewari-Singh, PhD

Assistant Research Professor, Department of Pharmaceutical Sciences


Mailing address:

University of Colorado
Skaggs School of Pharmacy and Pharmaceutical Sciences
Mail Stop C238
12850 E. Montview Blvd. V20-2430
Aurora, CO 80045

Office Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
2nd Floor
Room 2119

Lab Location:

Pharmacy and Pharmaceutical Sciences Building (V20)
2nd Floor
Room 2430

Contact:

Affiliation:

NIH Countermeasures Against Chemical Threats (CounterACT) program, Denver Center of Excellence

Training and Education:

  • BS, Lucknow University, India (Chemistry, Biology)
  • MS, Jawaharlal Nehru University, New Delhi, India (Life Sciences)
  • PhD, Jawaharlal Nehru University, New Delhi, India and University of Hannover, Germany (Molecular Biology and Genetic Engineering)
  • Post-Doctoral Fellow, Colorado State University
  • Research Associate, University of Colorado Denver

Research Interests:

The long-term goal of my research is to develop medical countermeasures against the threat of chemical agent exposures to the human population, and enhance the medical response capabilities in case of an emergency/warfare/terrorist activity. Under the CounterACT program with Dr. Rajesh Agarwal, current focus of our research is to identify mechanism-based therapeutics against skin and ocular injuries following exposure to mainly vesicating chemical warfare agents, such as sulfur mustard, nitrogen mustard and lewisite. Accordingly, our efforts are directed towards the development of relevant animal models of toxicity for gaining insight into the mechanism of action of vesicating agents to eventually tailor interventions that target the relevant pathways involved in the related lesions (mainly DNA damage, oxidative stress, inflammation and vesication) to the skin and ocular tissues.

Representative Publications:

  • Tewari-Singh, N., Inturi, S., Jain, A. K., Agarwal, C., Orlicky, D. J., White, C. W., Agarwal, R., and Day, B. J. (2014) Catalytic antioxidant AEOL 10150 treatment ameliorates sulfur mustard analog 2-chloroethyl ethyl sulfide-associated cutaneous toxic effects. Free Radical Biology & Medicine, 72, 285-295.
  • Inturi, S., Tewari-Singh, N., Agarwal, C., White, C. W., and Agarwal, R. (2014) Activation of DNA damage repair pathways in response to nitrogen mustard-induced DNA damage and toxicity in skin keratinocytes. Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 763-764C, 53-63.
  • Jain, A. K., Tewari-Singh, N., Inturi, S., Orlicky, D. J., White, C. W., and Agarwal, R. (2014) Myeloperoxidase deficiency attenuates nitrogen mustard-induced skin injuries. Toxicology, 320, 25-33.
  • Tewari-Singh, N., Jain, A. K., Orlicky, D. J., White, C. W., and Agarwal, R. (2014) Cutaneous injury-related structural changes and their progression following topical nitrogen mustard exposure in hairless and haired mice. PloS One, 9, e85402.
  • Jain, A. K., Tewari-Singh, N., Inturi, S., Orlicky, D. J., White, C. W., and Agarwal, R. (2014) Histopathological and immunohistochemical evaluation of nitrogen mustard-induced cutaneous effects in SKH-1 hairless and C57BL/6 mice. Experimental and Toxicologic Pathology, 66, 129-138.
  • Inturi, S., Tewari-Singh, N., Jain, A. K., Roy, S., White, C. W., and Agarwal, R. (2013) Absence of a p53 allele delays nitrogen mustard-induced early apoptosis and inflammation of murine skin. Toxicology, 311, 184-190.
  • Tewari-Singh, N., Jain, A. K., Inturi, S., White, C. W., and Agarwal, R. (2013) Clinically-relevant cutaneous lesions by nitrogen mustard: useful biomarkers of vesicants skin injury in SKH-1 hairless and C57BL/6 mice. PloS One, 8, e67557.
  • Tewari-Singh, N., Jain, A. K., Inturi, S., Agarwal, C., White, C. W., and Agarwal, R. (2012) Silibinin attenuates sulfur mustard analog-induced skin injury by targeting multiple pathways connecting oxidative stress and inflammation. PloS One, 7, e46149.
  • Tewari-Singh, N., Jain, A. K., Inturi, S., Ammar, D. A., Agarwal, C., Tyagi, P., Kompella, U. B., Enzenauer, R. W., Petrash, J. M., and Agarwal, R. (2012) Silibinin, dexamethasone, and doxycycline as potential therapeutic agents for treating vesicant-inflicted ocular injuries. Toxicology and Applied Pharmacology, 264, 23-31.
  • Inturi, S., Tewari-Singh, N., Gu, M., Shrotriya, S., Gomez, J., Agarwal, C., White, C. W., and Agarwal, R. (2011) Mechanisms of sulfur mustard analog 2-chloroethyl ethyl sulfide-induced DNA damage in skin epidermal cells and fibroblasts. Free Radical Biology & Medicine, 51, 2272-2280.
  • Jain, A. K., Tewari-Singh, N., Gu, M., Inturi, S., White, C. W., and Agarwal, R. (2011) Sulfur mustard analog, 2-chloroethyl ethyl sulfide-induced skin injury involves DNA damage and induction of inflammatory mediators, in part via oxidative stress, in SKH-1 hairless mouse skin. Toxicology Letters, 205, 293-301.
  • Jain, A. K., Tewari-Singh, N., Orlicky, D. J., White, C. W., and Agarwal, R. (2011) 2-Chloroethyl ethyl sulfide causes microvesication and inflammation-related histopathological changes in male hairless mouse skin. Toxicology, 282, 129-138.
  • Tewari-Singh, N., Agarwal, C., Huang, J., Day, B. J., White, C. W., and Agarwal, R. (2011) Efficacy of glutathione in ameliorating sulfur mustard analog-induced toxicity in cultured skin epidermal cells and in SKH-1 mouse skin in vivo. The Journal of Pharmacology and Experimental Therapeutics, 336, 450-459.
  • Tewari-Singh, N., Gu, M., Agarwal, C., White, C. W., and Agarwal, R. (2010) Biological and molecular mechanisms of sulfur mustard analogue-induced toxicity in JB6 and HaCaT cells: possible role of ataxia telangiectasia-mutated/ataxia telangiectasia-Rad3-related cell cycle checkpoint pathway. Chemical Research in Toxicology, 23, 1034-1044.
  • Pal, A., Tewari-Singh, N., Gu, M., Agarwal, C., Huang, J., Day, B. J., White, C. W., and Agarwal, R. (2009) Sulfur mustard analog induces oxidative stress and activates signaling cascades in the skin of SKH-1 hairless mice. Free Radical Biology & Medicine, 47, 1640-1651.
  • Tewari-Singh, N., Rana, S., Gu, M., Pal, A., Orlicky, D. J., White, C. W., and Agarwal, R. (2009)< Inflammatory biomarkers of sulfur mustard analog 2-chloroethyl ethyl sulfide-induced skin injury in SKH-1 hairless mice. Toxicological Sciences, 108, 194-206.