MAVRC Flow Cytometry Core Facility
The MAVRC/Denver VA Flow Cytometry Core is a user-supported facility purchased by the VA (VA Eastern Colorado Health Care System) in January 2009. Located in Building 23 at the Denver Veteran's Administration, 1055 Clermont Street, the 3-laser instrument evaluates from 1-14 colors, size and granularity of cells.
After initial training, the instrument can be operated successfully by laboratory personnel without a core operator, although assistance is available from the Core Supervisor.
LSR II Flow Cytometer 3-Laser Configuration:
Each lettered circle represents a photomultiplier tube (PMT). The rectangle closest to the PMT is the "high pass" filter that shows the range of wavelengths passing through the filter (e.g., 780/60 = 750-810l). The lower rectangle is the "long pass" filter that shows the highest wavelength emitted to the next alphabetical PMT (e.g, 755 LP). Additional filters: 635 LP; 600LP; 670/14; 610/20; and 585/42.