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Real Time Quantitative PCR

Gene Expression and Genotyping


 

Goal:  To provide real time quantitative PCR services, including gene expression and copy number variation (CNV) analysis.  The core also offers support services such as RNA and DNA purification and DNAse treatment.  Gene expression analysis is done using the relative standard curve method and an appropriate endogenous control.  This ensures the most accurate data possible that will stand up to current publishing recommendations.  Copy number analysis can be applied to test for transgene copy number in addition to inherent genomic variations.  The core uses Applied Biosystems 7500Fast instrument to deliver reliable data in a timely manner.  We can customize projects to meet your research and budget needs.

Contact: Kris Williams, BS

 
Real Time QPCR - Gene Expression
Member: Price per Plate: $193.82, Price per Sample: $18.68
Non member: Price per Plate: $455.73, Price per Sample: $18.68
 ABI 7500Fast

The listed price is for initial consultation with client, reverse transcription and gene expression of your samples using real time PCR and data analysis and review.  The assay (primer/probe) for your gene of interest is NOT included.  This must be ordered separately by the client.  All samples are run in triplicates.

The Plate Set-up price includes the set up of a 96-well plate, with all necessary controls.  To the Plate Set-up Price add the Price per Sample based on how many samples you will be running.  A maximum of 8 samples can be run on a 96-well plate.  Another Plate Set-up fee will be charged for each additional 96-well plate needed to accommodate your number of samples.  The prices above apply to testing one gene.  If you want to test multiple genes, multiply the prices by the number of genes.
 

Real Time QPCR - CNV

Member: Price per Plate: $106.37, Price per Sample: $4.12
Non Member: Price per Plate: $237.32, Price per Sample: $4.12

The Plate Set-up price is for initial consultation with client, analysis of your samples and data review.  The assay (primer/probe) for your gene of interest is NOT included.  This must be ordered separately by the client.  To the Plate Set-up Price add the Price per Sample based on how many samples you will be running.  A maximum of 11 samples can be run on a 96-well plate.  Another Plate Set-up fee will be charged for each additional 96-well plate needed to accommodate your number of samples.  All samples are run in triplicates.


Support Services

To the Set-up Price add the Price per Sample based on how many samples you will be running.

DNA Purification
Member: Set-up Price: $30.52, Price per Sample: $5.35
Non Member: Set-up Price: $117.82, Price per Sample: $5.35

RNA Purification
Member: Set-up Price: $30.52, Price per Sample: $8.56
Non Member: Set-up Price: $117.82, Price per Sample: $8.56

DNAse Treatment
Member: Set-up Price: $15.26, Price per Sample: $7.59
Non Member: Set-up Price: $58.91, Price per Sample: $7.59

 

*Prices are valid for the 2014 fiscal year
*Member prices apply to the members of the Center for Neuroscience.  For information on how to become a member, fill out the attached form: Membership Form
*For clients outside of the University of Colorado, add another 10% to the Non Member prices

 

Contact the Colorado IDDRC

Phone: 303-724-3839 • Fax: 303-724-3838

Mailing Address: Mail Stop 8313, 12800 E. 19th Avenue • Aurora, CO 80045

E-mail: Peds.IDDRC@ucdenver.edu

University of Colorado Denver

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