technologies are rapidly advancing our ability to detect RNA molecules, with huge
increases in speed and sensitivity. Unfortunately, these new technologies are outstripping the
ability of any single laboratory to analyze and identify new insights from the data. To
bridge this gap, the RNA Bioscience Initiative is creating a variety of training opportunities to
broaden the capabilities of researchers in the Center and
in the Anschutz community.
The RNA Bioscience Initiative will sponsor the Genome Analysis Workshop, a hands-on tutorial of skills needed to
process large genomics data sets and visualize their results. The class is
taught from the standpoint of a biologist with practical goals (e.g., to
interpret the results of a sequencing-based experiment and gain
biologically meaningful insight). The class focuses on working in the UNIX/Linux environment, with emphasis on
command-line tools, Python programming, and the R statistical computing
We use publicly available next-generation DNA sequencing data
from the ENCODE project to illustrate standard approaches for manipulating
sequencing data, aligning sequences to a reference genome, generating
coverage plots, and displaying them in the UCSC Genome Browser. We will
cover specific analyses used in ENCODE project including ChIP-seq, mRNA-seq, and genome sequencing to identify single nucleotide
The workshop is currently offered in the Spring semester.
The Initiative will offer several short courses focused on RNA technology and informatics. We will host 1-2 week intensive courses on select topics including:
- Chromatin Immunoprecipitation of RNA-related factors (ChIP-seq)
- Nascent RNA analysis (BRU-seq, GRO-seq)
- Analysis of transcription and translation by polymerase and ribosome profiling
We anticipate the first technology workshops to be held in Summer 2017.
The RNA Bioscience Initiative will provide fellowship opportunities for trainees at several levels:
We anticipate the Scholars and Fellows program to be initiated for 2016-2017, and the first Interns will visit in Summer 2017.
- Undergraduate "RNA Interns" will spend 10 weeks in the summer on an Initiative-sponsored project.
- Graduate student "RNA Scholars" will receive 1 year stipends to enhance their study of RNA.
- Post-doctoral "RNA Fellows" will include support for experimental and computational fellows.