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New technologies are rapidly advancing our ability to detect RNA molecules, with huge increases in speed and sensitivity. Unfortunately, these new technologies are outstripping the ability of any single laboratory to analyze and identify new insights from the data. To bridge this gap, the RNA Bioscience Initiative is creating a variety of training opportunities to broaden the capabilities of researchers in the Center and in the Anschutz community.

Graduate Courses

The RNA Bioscience Initiative will sponsor the Genome Analysis Workshop, a hands-on tutorial of skills needed to process large genomics data sets and visualize their results. The class is taught from the standpoint of a biologist with practical goals (e.g., to interpret the results of a sequencing-based experiment and gain biologically meaningful insight). The class focuses on working in the UNIX/Linux environment, with emphasis on command-line tools, Python programming, and the R statistical computing environment. 

We use publicly available next-generation DNA sequencing data from the ENCODE project to illustrate standard approaches for manipulating sequencing data, aligning sequences to a reference genome, generating coverage plots, and displaying them in the UCSC Genome Browser. We will cover specific analyses used in ENCODE project including ChIP-seq, mRNA-seq, and genome sequencing to identify single nucleotide variants. 

The workshop is currently offered in the Spring semester.

Technology Workshops

The Initiative will offer several short courses focused on RNA technology and informatics. We will host 1-2 week intensive courses on select topics including:

  • Chromatin Immunoprecipitation of RNA-related factors (ChIP-seq)
  • Nascent RNA analysis (BRU-seq, GRO-seq)
  • Analysis of transcription and translation by polymerase and ribosome profiling
We anticipate the first technology workshops to be held in Summer 2017.

Training Fellowships

The RNA Bioscience Initiative will provide fellowship opportunities for trainees at several levels:

  • Undergraduate "RNA Interns" will spend 10 weeks in the summer on an Initiative-sponsored project.
  • Graduate student "RNA Scholars" will receive 1 year stipends to enhance their study of RNA.
  • Post-doctoral "RNA Fellows" will include support for experimental and computational fellows.
We anticipate the Scholars and Fellows program to be initiated for 2016-2017, and the first Interns will visit in Summer 2017.