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James L. McManaman, Professor

Ph.D. (1978) University of Colorado, Boulder


Departments of Obstetrics &Gynecology, Physiology and Biophysics
Chief, Section of Basic Reproductive Sciences
 
Campus Box 8309
RC-1 North , P18-5104
Phone: 303-724-3500
Fax     303-724-3512

Jim.McManaman@UCDenver.edu

The McManaman laboratory is interested in four general areas:

(1) The molecular process by which cells accumulate and secrete triglycerides. This area of research focuses on role of members of the PAT family of lipid droplet binding proteins and directed at understanding how these proteins function to stabilize triglyceride at the molecular and structural levels;


(2) The physiological mechanisms controlling triglyceride mobilization from adipose stores during pregnancy and lactation. This area of research focuses on paracrine and hormonal mechanisms regulating triglyceride mobilization from adipose depots during pregnancy and lactation with special emphasis on mechanisms of cross-talk between adipose and epithelial compartments of the mammary gland.

(3) The physiological and molecular mechanisms underlying normal metabolic adaptations to lactation and the effects of obesity on this process. This research focuses on identifying distinct dietary and obesigenic mechanisms.

(4) Pathological mechanisms controlling premature birth. This project is focused on defining the changes in the human vaginal fluid proteome during pregnancy and identifying differences in the vaginal fluid protein composition of women delivering at term and those delivering preterm.

Dr. McMananam was recently inteviewed by local media regarding the adipophilin (PLIN2) knockout mouse, which fails to gain weight and appears healthy, even on a high fat diet:

CU Researchers slim down mice by deleting obesity gene​

Denver Post: Fat Cure?​

Obesity Gene​

CU researchers one step closer to combating obesity​

(1) Russell, T.D., Palmer, C.A., Orlicky, D.J., Bales, E.S., Chang, B. H-J., Chan, L. and McManaman, J.L. Mammary glands of adipophilin-null mice produce an N-terminally truncated form of adipophilin that mediates milk lipid formation and secretion. J. Lipid Res. 49: 206-216, 2008
(2) Klein, L.L., Jonscher, K.R., Heerwagen, M.J., Gibbs, R.S. and McManaman, J.L., Shotgun proteomic analysis of vaginal fluid from women in late pregnancy. Reproductive Sciences 15: 263-773, 2008
(3) Orlicky, D.J., Degala, G. Greenwood, C., Bales, E.S., Russell, T.D. and McManaman, J.L. Multiple Functions Encoded by the N-terminal PAT domain of Adipophilin, J. Cell Sci. 121: 2921-2929, 2008
(4) McManaman, J.L. Formation of Milk Lipids: Molecular Perspective. Clinical Lipidology 4: 391-401, 2009
(5) McManaman. J.L., Palmer, C.A., Anderson S., Schwertfeger, K. and Neville M.C. Regulation of milk lipid formation and secretion in the mouse mammary gland. Adv Exp Med Biol., 554, 263-279, 2004
(6) Wang, Z., Jiang, T., Li, J., Proctor,G., McManaman, J.L., Lucia, S., Chua, S. and Levi, M. Accumulation and glomerulosclerosis in FVBdb/db mice with type II diabetes mellitus. Diabetes 54:2328-35, 2005
(7) Palmer, C.A., Neville, Anderson, S.M. and McManaman, J.L. Analysis of lactation defects in transgenic mice. J Mammary Gland Biol Neoplasia. 11:269-82, 2006
(8) Rudolph, M.C., McManaman, J.L., Serkova, N., Russell, T.D., Phang, T., Anderson, S.M. and Neville, M.C. Metabolic regulation in the lactating mammary gland: A lipid synthesizing machine, Physiol. Genomics 28:323-336, 2007
(9) Anderson, S.M., Rudolph, M.C., McManaman, J.L and Neville, M.C. Key stages in mammary gland development. Secretory activation in the mammary gland: it's not just about milk protein synthesis. Breast Canc. Res. 9: 204-218, 2007
(10 Schroeder-Gloeckler, J.M., Rahman, S.M., Janssen, R. C., Qiao, L., Shao, J., Roper, M., Fischer, S.J., Lowe, E., Orlicky D.J., McManaman, J.L., Palmer, C., Gitomer, W.L., Huang. Wan, O’Doherty, R.M., Becker, T.C., Klemm, D.J., Jensen D.R., Pulawa, L.K., Eckel, R.H. and Friedman, J.E. CCAAT/enhancer-binding protein beta (C/EBPbeta ) deletion reduces adiposity, hepatic steatosis, and diabetes in Leprdb/db mice. J.Biol.Chem. 282: 15717-15729, 2007
(11)Harvell, DM, Spoelstra, NS, Singh, M, McManaman, JL, Finlayson, C, Phang, T, Trapp, S, Hunter, L, Borges. VF, Elias, A. Horwitz, KB and Richer, JK Molecular signatures of neoadjuvant endocrine therapy for breast cancer: characteristics of response or intrinsic resistance. Breast Cancer Res Treat (epub) 2008
(12) Watkin, HL, Richert, MM, Lewis, A, Terrell, K, McManaman, JL and Anderson, SM Lactation failure in Src knockout mice is due to impaired secretory activation. BMC Dev Biol 23: 6, 2008


Latest Publications in PubMed