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Rates and Fee Structures


Prices below are subsidized by the Human Immunology Immunotherapy Dean’s Transformation Initiative (CU School of Medicine).  Further reduced rates are available for preliminary data that will be used in future grant applications, please inquire if your study qualifies. 
Users outside the CU system, please inquire about non-academic, non-subsidized pricing.

Please contact Kim Jordan (Kimberly.Jordan@ucdenver.edu) for help building a budget that includes HIMSR services in your grant applications.

EXPERIMENTAL DESIGN SUPPORT



description​

reagent fee​

service fee​

additional samples​
​Initial project design support ​Discussion of research endpoints, selection of appropriate assays and fee structure NA FREE​ NA​
​Experimental Design and Analysis Technical support, assay design, selection of appropriate markers, and bioinformatics support​ NA​ $40/hr​ NA​
​Ongoing experimental design support
Assay development, extensive analysis and interpretation, grant and publication writing support, extensive bioinformatics support NA​ % effort​ NA​
 

SAMPLE PROCESSING



description​


reagent fee​

service fee​

additional samples​*
​Blood cell isolation Ficoll gradient​ (per 3 blood tubes) $10
​$80 ​$10
​CPT tube $2 ​$40 ​$5
​RBC lysis $3 ​$40 ​$5
Plasma/Serum preparation​ ​Isolation and aliquot (cryopres. if needed) $3 ​$20 ​$5
​Tissue cell isolation Miltenyi gentleMACS​

​$7-$22, dependent on sample type ​$100 ​Reagent fee (up to 8)
​Manual Disruption (COMING SOON!)
​RNA/DNA isolation ​RNA/DNA kit $10 ​$40 ​$10
Transfer to ​Paxgene tube $10 ​$10 ​$15
*listed fees are for additional blood tubes and/or different study subjects submitted at the same time  

CELL SORTING


description​​​

reagent fee​

service fee​

additional samples*
​BD FACSAria Fusion ​Sample staining $2/marker​ $80​ $2/marker​
Sample run time (per hour)​ included​ $150/hour​
​Miltenyi AutoMACS ​COMING SOON!
*listed fees are for additional samples stained at the same time

MASS CYTOMETRY

CyTOF​



description​​


reagent fee​

service fee​

additional samples*​
Reagents Surface marker antibodies
Intracellular marker antibodies
​$3.50/test
$7/test
NA
NA​
NA
NA​
Sample Staining ​Phenotype panel $3/marker $80​ $3/marker
​Signaling panel $6/marker $80​ ​$6/marker
​Cytokine panel ​$6/marker ​$80 ​$6/marker
​Custom panel development or substitutions $25/antibody​ ​$80
​Sample barcoding ​$15/barcode $80 $15​
​Sample run ​Sample run/hour at Cancer Center Flow Core included​ $140/hour​
​Sample analysis
​Data normalization, barcode unmixing, cellular phenotyping and marker expression, data export to spreadsheet NA​ ​$40/hour NA​
*listed fees are for additional samples stained at the same time

FLOW CYTOMETRY

Fortessa X-20





description​​




reagent fee​




service fee​



additional samples​*
​Sample staining ​phenotype panel ​$2/marker $80​ $2/marker​
​signaling panel $4/marker​ $80​ $4/marker
​cytokine panel $4/marker
$80​ $4/marker
​Custom panel development or substitutions $25/antibody​ $80/antibody​
​Sample run ​Sample run/hour included​ $150/hour​
​Sample analysis
​up to 8 colors, phenotyping only NA​ ​$80 ​$1
​9+ colors, phenotyping only ​NA ​$100 ​$1
​Up to 8 colors, phenotyping with marker expression MFI
​NA ​$100 ​$1
​9+ colors, phenotyping with marker expression MFI ​NA ​$120 ​$1
*listed fees are for additional samples stained at the same time

IMAGING

Vectra 3.0



description​


reagent fee​

service fee

additional slides​*
​Reagents ​Primary antibodies 5ul aliquot​ dependent on price ​NA
​Perkin Elmer secondary polymer reagent (anti-mouse/anti-rabbit)
Perkin Elmer antibody diluent/blocking reagent
Perkin Elmer OPAL fluorescent reagent
$0.70/slide/color

$0.30/slide/color

$3/slide/color​
​Scanning Slide scanning ​(manual single color acquisition, unstained controls, 10X slide scan and/or 20X multispectral scans) NA​ $60/hr $5​
​Staining ​Slide staining Leica Autostainer** (investigator provides FFPE tissue slides, standard HIMSR panels) ​7 color $45/slide
6 color $40/slide
5 color $35/slide
4 color $30/slide
$10
$10
$10
$10
NA
​Custom panel development or substitutions ​At cost or provided by investigator​ $180/antibody​ ​ NA
inForm software analysis
(each higher level of service includes the analysis above it)
​inForm software self-analysis and training ​FREE
​Images only (no quantification) ​NA $50​ $5​
​Tissue segmentation (e.g. tumor v. stroma) NA​ $50​ ​$5
​Cell segmentation (staining intensity) ​NA ​$120 ​$15
​Phenotyping (cell numbers, intensity) ​NA ​$140 ​$20
​Scoring (cell numbers, intensity) ​NA ​$160 ​$25
Nearest neighbor (spatial relationships) NA $250 Project dependent
*listed fees are for additional slides stained at the same time or analyzed in the same project. Bulk pricing is available for slide scanning.
**limit of 30 slides per batch


IMMUNOASSAYS


description​​

reagent fee​

service fee​

additional samples​
​MesoScale Discovery Parsec ​Multiplex protein arrays
​~$140/analyte/
plate
​$160/plate set ​Reagents
(up to 4 plates/set)
​Luminex ​Up to 500 analytes
​Dependent on analyte ​$160/plate set ​Reagents
(up to 4 plates/set)
​ELISA ​Single analyte detection
​Dependent on analyte ​$160/plate set ​Reagents
(up to 4 plates/set)
 

IMMUNE FUNCTION



description​​

reagent fee​

service fee​


additional samples​
​Ex vivo stimulation ​Mitogen or peptide stimulation ​$15/stimuli ​$80/sample
​ELISPOT IFNg or Granzyme B read-outs
​$20/plate ​$80/sample
​Ex vivo killing assay COMING SOON!
​Ex vivo proliferation assay COMING SOON!
​SMART tube cell stimulation
​Whole blood stimulation in the SMART tube base station $25/tube​ $20​ $5 (max of 5 tubes per batch)​
 

DATA ANALYSIS



description​

reagent fee​

service fee​

additional samples​
inForm software​ ​Custom inForm analysis NA
See imaging above
See imaging above​
​Spotfire software ​COMING SOON!
​Cytobank ​Self-analysis station located in the Immuno&Micro HIMSR Flow Core ​FREE
​FlowJo ​Self-analysis station located in the Immuno&Micro HIMSR Flow Core ​FREE

​​​​​