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Imaging

Identify and localize immune cells in the microenvironment of your tissues samples


Are you interested in quantitating immune cells that infiltrate the human tissues you study, understanding where they are located, or determining which other cells they are near in the tissue architecture? HIMSR is equipped to help using Perkin Elmer’s Vectra® 3, an automated microscope that images brightfield or immunofluorescence slides. With this instrument, we can distinguish nuclear DNA plus up to 6 different cellular markers. We have pre-developed antibody panels​ or can help develop new antibodies to help you analyze your tissues.

This technology is optimal for analysis of formalin-fixed, paraffin-embedded tissues or tissue microarrays (not frozen tissues). The antibodies are resolved with Perkin Elmer Opal technology, which allows use of the best primary antibodies for immunohistochemistry, regardless of species with no cross-reactivity. Log into iLabs for fees; contact Kim Jordan for a quote.

Vectra® 3
For every antibody panel, we include stained tonsil-tissue controls, scan slides with a 10x, 20x, or 40x objective, and can provide analysis depending on your needs.


Opal technology
Opal technology includes 6 fluorophors (and enhanced DAPI to stain DNA) which are read at different wavelengths. Briefly, we apply a primary detecting antibody, a secondary antibody, the Opal reagent, microwave to remove the antibodies but not the Opal reagent, repeat.
​inForm software
inForm is a patented image analysis software package that uses trainable algorithms in a user-friendly interface to accurately visualize and quantify biomarkers in tissue sections. Capability includes phenotyping, co-localization, co-expression, tissue region detection (e.g. tumor versus stroma), and traditional scoring functions.  This software is available for use on a dedicated analysis station within the HI3 HIMSR. [Coming soon! Check the videos from local workshops on how to use this software.]



Leica Autostainer
Using the Leica Autostainer, we have developed standardized antibody panels for the analysis of tissue microenvironments in human FFPE tissue for detection by the Vectra instrument.  The Leica Autostainer can perform the entire 7-color protocol on 30 slides in one overnight batch.  We can also run smaller batches of slides or multiple different panels at one time. If you wish to perform the staining in your own laboratory, we are happy to provide the antibody clone names and staining protocols.
 
Custom IF antibody panel
If your antibody panel of choice is not yet available, we can work with you to develop a custom human antibody panel either in your lab or HIMSR. In the spirit of fostering collaborative research at CU|AMC, antibody panels developed in HIMSR will be shared with the campus as future standard panels.