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Peptide Protein Chemistry Facility


The Peptide and Protein Chemistry Facility at the University of Colorado at the Anschutz Medical campus provides peptide synthesis, purification and peptide/protein composition analysis as well as mass spectrometry services for academic and industrial clients.

The Peptide and Protein Chemistry facility personnel help users choose and design experiments using appropriate chemistry/instrumentation, to obtain the necessary data, and interpret the results. The Facility also provides instruction for graduate students and postdoctoral fellows for the use of HPLC and LC/MS approaches.

The facility is equipped with state of the art instrumentation and technical support suitable for diverse applications and samples.


Beckman 6300 AA analyzer

Agilent 1100 HPLC systems

Agilent SL/XCT Electrospray MS systems

Advanced Chemtech peptide synthesizer

PEPTIDE SYNTHESIS

  • Minimum charge is for 10 residues ($250)
  • 0.1 mM scale (50-100 mg crude peptide): $25 per residue
  • 0.25 mM scale (100-300 mg crude peptide): $40 per residue
  • All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and mass spectrometry
  • Preparative HPLC purification of synthetic peptides: $100 per peptide
  • Additional modification of peptides:
    • phosphorylation: $100 - $150
    • acetylation: $15
    • C-terminal biotin: $175
    • fluorescent label: $200
    • N-terminal biotin: $100
    • methylation: $175 - $400

AMINO ACID ANALYSIS

  • $20 per sample

MASS SPECTROMETRY

  • Mass determination of proteins, peptides, etc: $15 per sample, $100/hour
  • LC/MS/MS analysis: $30 per sample, $100/hour
  • 2D LC/MS/MS analysis: $100/sample
  • Enzymatic digestion of proteins in gel slices and protein identification byLC/MS/MS: $200 - $500

Contact Information

Peptide and Protein Chemistry Facility 
University of Colorado Anschutz Medical Campus 
Bldg. RC-1 South, Room L18-1308 
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045

Dr. Robert S. Hodges, Director
voice: 303-903-5591
fax: 303-724-3249
Office: Fitzsimons, RC-1 South Tower, Room 9121
Robert.Hodges@ucdenver.edu

Dziuleta Cepeniene, Professional Research Assistant
voice: 303-724-3336
Office: Fitzsimons, RC-1 South Tower, Room 1308
Dziuleta.Cepeniene@ucdenver.edu