The Peptide and Protein Chemistry Facility at the University of Colorado at the Anschutz Medical campus provides peptide synthesis, purification and peptide/protein composition analysis as well as mass spectrometry services for academic and industrial clients.
The Peptide and Protein Chemistry facility personnel help users choose and design experiments using appropriate chemistry/instrumentation, to obtain the necessary data, and interpret the results. The Facility also provides instruction for graduate students and postdoctoral fellows for the use of HPLC and LC/MS approaches.
The facility is equipped with state of the art instrumentation and technical support suitable for diverse applications and samples.
Beckman 6300 AA analyzer
Agilent 1100 HPLC systems
Agilent SL/XCT Electrospray MS systems
Advanced Chemtech peptide synthesizer
PEPTIDE SYNTHESIS
- Minimum charge is for 10 residues ($250)
- 0.1 mM scale (50-100 mg crude peptide): $25 per residue
- 0.25 mM scale (100-300 mg crude peptide): $40 per residue
- All peptides are cleaved from the resin, resolubilized in appropriate solvent, and analyzed by reversed phase HPLC and mass spectrometry
- Preparative HPLC purification of synthetic peptides: $100 per peptide
- Additional modification of peptides:
- phosphorylation: $100 - $150
- acetylation: $15
- C-terminal biotin: $175
- fluorescent label: $200
- N-terminal biotin: $100
- methylation: $175 - $400
AMINO ACID ANALYSIS
MASS SPECTROMETRY
- Mass determination of proteins, peptides, etc: $15 per sample, $100/hour
- LC/MS/MS analysis: $30 per sample, $100/hour
- 2D LC/MS/MS analysis: $100/sample
- Enzymatic digestion of proteins in gel slices and protein identification byLC/MS/MS: $200 - $500
Contact Information
Peptide and Protein Chemistry Facility
University of Colorado Anschutz Medical Campus
Bldg. RC-1 South, Room L18-1308
12801 E. 17th Avenue, Mail Stop 8101
Aurora, CO 80045
Dr. Robert S. Hodges, Director
voice: 303-903-5591
fax: 303-724-3249
Office: Fitzsimons, RC-1 South Tower, Room 9121
Robert.Hodges@ucdenver.edu
Dziuleta Cepeniene, Professional Research Assistant
voice: 303-724-3336
Office: Fitzsimons, RC-1 South Tower, Room 1308
Dziuleta.Cepeniene@ucdenver.edu