Fee for Service – Rates
A charge is applied to all users of the Proteomic Facility to offset the running costs associated with maintaining this facility. Details of the fees are given in the following table.
|
|
Cancer Center
& CCTSI |
Academic |
External |
| Labor / Sample Prep / Data Analysis |
$28 / hour |
$35 / hour |
$50 / hour |
| Gel Set-Up |
$30 /gel |
$45 / gel |
$60 /gel |
| $10 / zip tip |
$15 / zip tip |
$25 / zip tip |
| LTQ LC-MS |
First Hour / Infusion |
$100 |
$120 |
$175 |
| |
Runs 2+ |
$60 / hour |
$75 / hour |
$115 / hour |
| FT-ICR LC-MS |
First Hour / Infusion |
$115 / hour |
$150 / hour |
$195 / hour |
| |
Runs 2+ |
$70 / hour |
$90 / hour |
$135 / hour |
| Orbitrap LC-MS |
First Hour / Infusion |
$135 / hour |
$180 / hour |
$220 / hour |
| |
Runs 2+ |
$80 / hour |
$110 / hour |
$150 / hour |
| MALDI |
|
$25 / hour |
$33 / hour |
$50 / hour |
| Typhoon |
|
$50 / hour |
$50 / hour |
$50 / hour |
Analyses performed by the Proteomic Facility vary in scope, complexity and cost. Below are some "typical" analysis and estimates of the total cost of the analysis. For many proteomic projects (especially those that are aimed at more than simple discovery and integrate validation) samples would need to be run in multiples resulting in higher costs. There is substantial savings associated with investigators performing the labor involved with image and data analysis. However, this only becomes cost effective if the investigator is already trained in this type of analysis or a long term project is being undertaken.
Representative Fees for “Typical” Analysis Types
Scenario #1: 4 Gel Bands dropped off for MS Analysis by a Cancer Center member. Experiment goal – identify proteins in the gel bands
Process: Sample Prep – wash gel bands, reduce, alkylate and perform digestion. Analyze digests on the LC-LTQ instrument – inject each band 2 times for a total of 8, 60 minute runs, Data Analysis – search data using in house software to match spectra to peptides, map to protein/s and perform follow up searches for modifications of interest.
Protein Identification From Gel Bands (4 Bands) |
|
Time / # of Runs |
Cost |
Fee |
Sample Prep Digestion |
2 hrs |
$28 / hour |
$56 |
LTQ LC-MS Analysis - 8 Hours |
1st Injection / Hour |
$100 / hour |
$100 |
|
7 Subsequent Hours |
$60 / hour |
$420 |
Data Analysis |
1 hr |
$28 / hour |
$28 |
Total |
$604 |
Scenario #2: Final elution sample from an IP protocol containing detergents and salts was dropped off for MS Analysis by an academic group that isn’t a Cancer Center or CCTSI member. Experimental goal – ID antigen to validate pull-down and identify potential antigen interacting partners in 13 bands.
NOTE: We highly recommend that you discuss sample prep with us before bringing samples. The above IP elution conditions are not ideal for maximizing success. We also recommend submission of control /mock IP samples be run at the same time, it is common to identify proteins that don’t show appreciable staining.
Process: Gel – remove detergents and salts from sample by running a PA-gel, Typhoon – image the gel to correctly identify bands of interest, Sample Prep – cut, wash, and digest gel bands, Orbitrap LC-MC Analysis – inject each band 2 times for a total of 26, 60 minute runs, Data Analysis – search data using in house software to match spectra to peptides, map to protein/s and perform follow up searches for modifications of interest.
Protein Identification From Dirty Sample In Solution (Includes Detergents / Salts) |
|
Time / # of Runs |
Cost |
Fee |
Gel |
1 gel |
$45 / gel |
$45 |
Typhoon |
0.25 hr |
$50 / hr |
$12.50 |
Sample Prep Digestion |
4 hrs |
$35 / hour |
$245 |
Orbitrap LC-MS Analysis – 26 hours |
1st Injection / Hour |
$180 / hour |
$180 |
|
25 Subsequent Hours |
$110 / hour |
$2,750 |
Data Analysis |
2 hrs |
$28 / hour |
$56 |
Total |
$3,288.50 |
*Please note, all charges will be discussed with you prior to sample submission. These fees can change according to the scope of your project and the extent of collaboration with the Proteomic Facility.