The Mass Spectrometry/Proteomics Facility is equipped to perform detailed proteomic studies. Major instrumentation in the facility include the following:
ThermoFisher LTQ Orbitrap Velos, linear ion-trap ion cyclotron resonance mass spectrometer. This instrument is used for proteomic applications, including protein identification and detailed peptide mapping. Identification of proteins is performed on proteolytic digests by means of nanoflow LC-ESI-MS/MS with subsequent database searches using software packages such as Mascot and Protein Prospector. This instrument is capable of electron transfer dissociation which is useful for analysis of many post-translational modifications. This instrument was funded by the National Institutes of Health-National Center for Research Resources (NCRR).
ThermoFisher LTQ FT Ultra, linear ion-trap ion cyclotron resonance mass spectrometer. This instrument is capable of the two alternative activation stratagies electron capture dissociation (ECD) and infrared multiphoton dissociation (IRMPD). THis instrument was funded by the National Institutes of Health- National Center for Research Resources (NCRR). To learn more about this instrument and its capabilities you can visit the Thermo Scientific website.
Perseptive Biosystems Voyager STR+ MALDI-TOF mass spectrometer. This matrix assisted laser desorption ionization time-of-flight mass spectrometer with delayed extraction (time-lag) uses reflectron ioin optics for improved ion focusing (to improve signal resolution). This system provides an expeditious approach to qualitative (and quantitative) analysis of biopolymers. Currently this instrument is not performing to specifications - a new laser will be installed in February.
GE Healthcare, Life Sciences TyphoonTM Variable Mode Imager featured with versatile system platform, handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays. Consists with powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets. Red, green, and blue excitation wavelengths and a wide shoice of emission filters enable imaging of an extensive variety of fluorophores. Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.
If data that was collected on the FT instrument is used for publication please include the following acknowledgement:
The project described was supported by Grant Number S10RR023015 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH.