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Fluorescence Spectroscopy


 

Fluorescence is a luminescence caused by the emission of a photon upon the relaxation of an electron in an excited state to its ground state. Fluorescence spectroscopy, the detection of this phenomenon, is a powerful technique for biological applications as it can be used quantitatively and is a more sensitive technique than absorbance. The wide variety of fluorescent molecules available commercially makes this technique widely applicable to many systems.

Applications:

  • Quantitative detection of biomolecules
  • Studies of biomolecular structure

Instrument Info: Fluoromax-3 (Horiba Jobin Yvon, Installed May 2005)
Both the Polarizer and Plate Reader Accessories are installed on this instrument

Sample Requirements
         The standard rectangular cell takes approximately 1mL to fill.
The 96-well plates for the reader are available for purchase from the core and each well has a volume of ~200uL
Users are welcome to bring and use their own cuvettes.  

Training

Training consists of a basic walkthrough of the instrument conducted by core facility personnel.