Fluorescence is a luminescence caused by the emission of a photon upon the relaxation of an electron in an excited state to its ground state. Fluorescence spectroscopy, the detection of this phenomenon, is a powerful technique for biological applications as it can be used quantitatively and is a more sensitive technique than absorbance. The wide variety of fluorescent molecules available commercially makes this technique widely applicable to many systems.
- Quantitative detection of biomolecules
- Studies of biomolecular structure
Instrument Info: Fluoromax-3 (Horiba Jobin Yvon, Installed May 2005)
Both the Polarizer and Plate Reader Accessories are installed on this instrument
The standard rectangular cell takes approximately 1mL to fill.
The 96-well plates for the reader are available for purchase from the core and each well has a volume of ~200uL
Users are welcome to bring and use their own cuvettes.
Training consists of a basic walkthrough of the instrument conducted by core facility personnel.