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Title

Developmental Pathway to Psychosis

Status

Open

Project Description

The Maclean lab studies the etiology and pathogenesis of cystathionine beta-synthase deficient homocystinuria (CBSDH), Down syndrome (DS) and Fragile X syndrome (FXS). All three of these conditions involve a component of cognitive impairment for which the mechanisms are unknown and current treatment is either lacking or non-existent.  Our efforts focus a range of genomic, proteomic, biochemical, behavioral, genetic and molecular techniques upon mouse models of these diseases with a view towards delineating pathogenic mechanisms and the rational design of novel treatment strategies. 
With regard to CBSDH we have generated a novel transgenic mouse model of the disease  and using behavioral analysis, hippocampal microarrays and proteomic analysis we have uncovered a number of novel pathogenic mechanisms that we have subsequently confirmed in human  CBSDH tissue samples. This work has lead to the discovery of a novel treatment for CBSDH, for which a limited clinical trial will start at the Children’s hospital in Denver in February 2009.
In our work on DS we have used a number of behavioral tests to investigate the effects of choline supplementation upon cognitive function in the Ts65Dn mouse model of the disease. We have found that while perinatal treatment with choline is unable to improve the ability of these mice to form a conditioned taste aversion (a functional test of learning and memory in mice) this treatment does ameliorate attentional dysfunction. This work is now being extended into the use of anticholinesterase inhibitor drugs in pre-clinical studies using the Ts65Dn mouse model. In addition to this work, we have been involved in a collaborative project with Dr Kim Bjugstad’s laboratory investigating the possible application of mouse neural stem cell therapy to improving cognition in Ts65Dn mice.
Our studies on FXS have used behavioral and biochemical investigations of the FMRP1 knockout mouse model to investigate the hypothesis that aberrations in the hypothalamus-pituitary-adrenal axis  HPA axis contribute to the clinical sequela observed in humans with FXS. We observed no significant changes in corticosterone or expression level/location of the glucocorticoid receptor as a consequence of knocking out the FMRP1 gene indicating that this is probably not a promising therapeutic avenue for this condition.  Working in collaboration with Barbera Strupp at Cornell, we were able to show that the FMRP1 mouse does suffer from increased attentional dysfunction and is thus a suitable model for testing novel therapeutic strategies.

Mentor Name

Maclean, Ken

Mentor Location

RC1-N, room 4124

Mentor Contact Number

303-724-3818

Mentor Contact Email

ken.maclean@ucdenver.edu

Specialty

Enter Choice #1

Preceptor Name

 

Student

 

Department

Pediatrics

Funding Department/Program

Pediatrics

Area of Study

Developmental Neuroscience, Brain and Behavior - Child

Populations

 

Method(s)

Animal Models; Cell Biology, Molecular Biology, and Genetics

Disease or Symptom

Basic Human Processes; Mental Illness and Developmental Disabilities; Neurological Illness

Faculty/Lab Website

 

Display on public listings?

No

Attachments

Created at 9/3/2010 9:37 AM by Vetter, Stephanie
Last modified at 12/5/2012 10:18 AM by Watts, Katie