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Title

Antigen Identification in Multiple Sclerosis

Status

Open

Project Description

My research investigates the specificity of IgG in patients with multiple sclerosis (MS) using phage-displayed peptide libraries approach. A hallmark of MS is the persistence of oligoclonal IgG and elevated numbers of B cells in the CNS. Our published studies have demonstrated the antigen-driven response of clonally expanded B cells in MS. We are using recombinant antibodies generated from these B cells to identify peptide epitopes/mimotopes by panning phage-displayed random peptide libraries. The specificity of the peptides is confirmed by ELISA, immunoblots and competitive inhibition assays. By applying a highly sensitive phage mediated immuno-PCR technique, these peptides are screened for bindings to IgG in multiple MS patients. MS peptides can then be used to determine the corresponding protein antigens using bioinformatics approach. Identification of MS antigens has the potential to determine the cause of disease, and to develop strategies for diagnostic and therapeutic intervention.

Mentor Name

Yu, Xiaoli

Mentor Location

AMC RCII, Room 5007

Mentor Contact Number

303-724-4316

Mentor Contact Email

Xiaoli.yu@ucdenver.edu

Specialty

Enter Choice #1

Preceptor Name

 

Student

 

Department

Neurology

Funding Department/Program

Neurology

Area of Study

Immunology and Autoimmune Diseases; Neuroscience, Brain and Behavior - Adult

Populations

 

Method(s)

Hormonal

Disease or Symptom

Multiple Sclerosis; Neurological Illness

Faculty/Lab Website

http://www.ucdenver.edu/academics/colleges/medicalschool/departments/neurology/research/Pages/research.aspx#tab-5

Display on public listings?

No

Attachments

Created at 7/16/2010 4:26 PM by Odom, Joshua
Last modified at 12/5/2012 10:21 AM by Watts, Katie