Diagram of Projects and Cores
In this cycle we assess the key observation made from patient data analyses that lung injury precedes all other organ dysfunctions. Previous work by the HS Core also implicates massive transfusion as a key predictor of organ dysfunction. Analyzing the voluminous data obtained from mass spectrometric analysis of blood products, Project II (Silliman) focuses on select protein and lipid candidates as plausible mediators of lung injury. At the same time, animal experiments conducted by Project I (Moore) implicate the post shock mesenteric lymph as a causal factor in lung injury. To combat the inflammatory mediators flowing acutely into the lung, Project III (Banerjee) examines the potential for anti-inflammatory therapy with hyperosmolarity delivered directly to the lung.
Administrative Core (Banerjee): This core is essential to support the entire structure of this Center. The particular challenges of this Core include increasing regulatory compliance and hurdling obstacles presented in integrating seamlessly three distinct institutions and five different departments. This Core handles all financial matters, personnel issues, purchasing, animal usage, safety and inventories for the Center.
Human Subjects Core (Moore): The Human Core is in the hands of an extremely talented and dynamic trio of investigators. Together, Drs. Moore, Johnson, and Sauaia are eager to continue the hypotheses generating and translational research functions of the Human Core as well as the coordination of standard protocols, recruitment /consent of subjects, as well as collection of samples and clinical data.
Cell Imaging & Proteomics Core (Banerjee): In addition to supplying cell lines and excellent imaging support, the Core will now also handle the significant proteomics duties for all three projects. Much of the staffing remains the same from the previous cycles, thus ensuring continuity. One of the goals over the last cycle was to implement full MS proteomics capability to this Core. Recent data outflow from mesenteric lymphs (both human and animal), various blood products, patient plasma and hyperosmotically altered IP samples is threatening our capacity to test abundant hypotheses. Dr. Hansen has worked brilliantly with prominent lung injury groups (Drs. Michael Matthay, Jeanine Wiener-Kronisch, UCSF) [28-30] pioneering MS-proteomics. He has been very successful in obtaining and using the latest improvements in ionization, quadrupole and ion cyclotron resonance technologies, but he is also a leader in the development of proteomics software suites. He will devote about 50% of his effort to the core, together with two dedicated post-doctoral fellows. He will also supply additional scientific effort to Project I and II with their complicated biological mixtures.