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Marilyne Coulombe Mansfield, PhD

Senior Research Associate CCTCARE and Gill Laboratory


Dr. Coulombe is the lead research associate with extensive experience in immunology and islet transplantation.  She completed her BS in Microbiology at the University of Alberta, Edmonton, Alberta, Canada in 1983.  In 1987 she graduated with her MS, Experimental Medicine also at the University of Alberta.  In extramural status from the University of Alberta and in conjunction with the Barbara Davis Center of Childhood Diabetes completed her PhD in 1993, Medical Sciences/Medicine. 

Currently, Dr. Coulombe provides direct supervision for the CCTCARE laboratory group and the organization, and required training of faculty, trainees, and staff supported by the Center.  Secondary duties include conducting individual research projects in the fields of transplantation and/or autoimmunity.

Phone: 303.724.7625
Fax: 303.724.7624
Email: marilyne.coulombe@ucdenver.edu

1. Coulombe M, Warnock GL, Rajotte RV. Prolongation of islet xenograft survival by cryopreservation. Diabetes 36:1086-1088, 1987.

2. Coulombe M. Cryopreservation and immuno-alteration of transplanted rat islets of Langerhans. University of Alberta M.Sc. thesis, 1987.

3. Coulombe M, Warnock GL, Rajotte RV. Reversal of diabetes by transplantation of cryopreserved rat islets of Langerhans to the renal subcapsular space. Diabetes Research 8:9-15, 1988.

4. Rajotte RV, Warnock GL, Coulombe M. Islet cryopreservation: methods and experimental results in rodents, large mammals and humans. In: Transplantation of the Endocrine Pancreas. Van Schilfgaarde R and Hardy MA (eds). Elsevier Amsterdam, 1988

5. Rajotte RV, Warnock GL, Coulombe M. Islet isolation and preservation. In: Transplantation of the Endocrine Pancreas. Van Schilfgaarde R and Hardy MA (eds). Elsevier Amsterdam, 1988

6. Coulombe M, Warnock GL, Rajotte RV. Transplantation of cryopreserved islets of Langerhans beneath the kidney capsule of diabetic rats. Clin Invest Med 10:R-113, 1987.

7. Coulombe M, Hao L, Calcinaro F, Gill RG, Eugui EM, Allison AC, Lafferty KJ. Tolerance induction in adult animals: Comparison of RS-61443 and anti-CD4 treatment. Transplant Proc 23 (Suppl 2): 31-32, 1991

8. Coulombe M, Lafferty KJ, Gill RG. Long-term allograft acceptance in the absence of clonal deletion/anergy. J Cell Biochem (Suppl 15A): 276, 1991.

 

9. Gill RG, Coulombe M. Rejection of pancreatic islet xenografts does not require CD8+ T lymphocytes. Transplant Proc 24(6): 2877-2878, 1992.

10. Coulombe MG. Pancreatic Islet Immunogenicity in Immunity and Tolerance.  University of Alberta Ph.D. thesis, 1993.

11. Coulombe M, Gill RG. The nature of tolerance induction to peripheral (extrathymic) islet allografts. Transplant Proc 26(2):720-721, 1994.

12. Coulombe M, Gill RG. Tolerance induction to cultured pancreatic islet allografts. I. Characterization of the tolerant state. Transplantation 57(8):1195-1200, 1994.

13. Coulombe M, Gill RG. Tolerance induction to cultured pancreatic islet allografts. II. The status of anti-donor reactivity in tolerant animals. Transplantation 57(8):1201-1207, 1994.

14. Gill RG, Coulombe M. Islet xenografting in autoimmune diabetes. Transplant Proc 26(3):1140, 1994.

15. Gill RG, Wolf L, Daniel D, Coulombe M. CD4+ T cells are both necessary and sufficient for islet xenograft rejection. Transplant Proc 26(3):1203, 1994.

16. Wolf LA, Coulombe M, Gill RG. Donor antigen-presenting cell-independent rejection of islet xenografts. Transplantation 60(10):1164-1170, 1995.

17. Coulombe M, Gill RG. T lymphocyte indifference to extrathymic islet allografts. J Immunol 156: 1998-2003, 1996.

18. Gill RG, Coulombe M, Lafferty KJ. Pancreatic islet allograft immunity and tolerance: the two-signal hypothesis revisited. Immunol Rev 149: 75-96, 1996.

19. Coulombe M, Yang H, Guerder S, Flavell R, Lafferty KJ, Gill RG. Tissue immunogenicity: The role of MHC antigen and the lymphocyte costimulator B7-1. J Immunol 157: 4790-4795, 1996.

20. Stegall MD, Loberman Z, Ostrowska A, Coulombe M, Gill RG. Autoimmune destruction of islet grafts in the NOD mouse is resistant to 15-deoxyspergualin but sensitive to anti-CD4 antibody. J. Surg. Res. 64:156-160, 1996.

21. Coulombe M, Yang H, Gill RG. Adoptive transfer of CD4 T cell dependent allograft tolerance. Transplant Proc 29: 1166-1167, 1997.

22. Coulombe M, Yang H, Wolf LA, Gill RG. Tolerance to antigen-presenting cell-depleted islet allografts is CD4 T cell dependent. J. Immunol. 162:2503-2510. 1999.

23. Coulombe M, Nicolls MR, Yang H, Bolwerk A, Gill RG. Anti-LFA-1 therapy induces long-term islet allograft acceptance in the absence of IFN-g and IL-4. J. Immunol 164: 3627-3634, 2000.

    24. Nicolls MR, Coulombe M, Gill RG. The basis of immunogenicity of endocrine allografts. Critical Rev. Immunol. 21:87-101, 2001.

    25. Nicolls MR, Coulombe M, Diamond AS, Beilke J, Gill RG. Interferon-g is not a universal requirement for islet allograft survival. Transplantation 74: 472-477, 2002.

    26. Coulombe M, Nicolls MR, Beilke J, Gelhaus HC, Gill RG. CD4-dependent generation of dominant transplantation tolerance induced by simultaneous perturbation of CD154 and LFA-1 pathways. J. Immunol. 169:4831-4839, 2002.

    27. Coulombe M, Gill RG. Immunobiology of endocrine gland transplantation. In Williams & Wilkins 2002.

    28. Coulombe M, Gill RG. Tolerance induction to pancreatic islet allografts. Curr. Opin. Organ Transplant 9:258-263, 2004.

    30. Coulombe M, Gill RG. The immunobiology of pancreatic islet transplantation. Adv. Exp. Med. Biol. 552:154-169, 2004.

    31. Nakayama M, Beilke JN, Jasinski JM, Kobayashi M, Miao D, Li M, Coulombe MG, Liu E, Elliott JF, Gill RG, Eisenbarth GS. Priming and effector dependence on insulin B:9-23 peptide in NOD islet autoimmunity. J Clin Invest. 2007 Jul;117(7):1835-43.

    32. Coulombe M, Mann D, Yu L, Gill RG. MHC-matched bone marrow transplantation dominantly prevents autoimmunity but not autoreactivity. (Manuscript in preparation).

AWARDS:

Harry W. Bass Memorial Bursary, 09/1979

Alberta Heritage Fund for Medical Research Summer Studentship, 05/1983

National Science and Engineering Research Council Summer Studentship, 05/1983

Alberta Heritage Fund for Medical Research Graduate Studentship, 09/1983 -1986

J.B. Collip Diabetes Studentship, 09/1986 - 09/1989

Canadian Diabetes Association Graduate Studentship, 09/1989 - 06/1993

Canadian Governor General

Juvenile Diabetes Research Foundation Postdoctoral Fellowship, 1995-1997

Diabetes Endocrinology Research Center Pilot & Feasibility grant, 2000-2001