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McManaman Lab


​The Fate of Fat: Research in the McManaman laboratory investigates how cells store, metabolize, and traffic lipids at the molecular, structural, cellular and organ systems levels.  We use biochemical, molecular modeling, and live-cell imaging approaches to define the basic mechanisms by which cells store and traffic lipids.  We use mouse genetic models and systems biology approaches, including proteomics and metabolomics, to understand the molecular physiology of lipid storage, metabolism and secretion, and how dysregulation of these processes contribute to defects in neonatal metabolic health and metabolic disorders such as obesity.​
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Faculty

James McManaman, PhD, Professor and Director, Principal Investigator

Jenifer Monks, PhD, Instructor, Co-investigator

David Orlicky, PhD, Associate Professor, Co-investigator


Professional Research Assistants

Elise Bales, BS, Professional Research Assistant, Lab manager 


Students


  1. Orlicky, D.J., Degala, G. Greenwood, C., Bales, E.S., Russell, T.D. and McManaman, J.L. Multiple Functions Encoded by the N-terminal PAT domain of Adipophilin, J. Cell Sci. 121: 2921-2929, 2008
  2. Russell, T.D., Palmer, C.A., Orlicky, D.J., Bales, E.S., Chang, B. H-J. Chan, L. and McManaman, J.L. Mammary glands of adipophilin-null mice produce an N-terminally truncated form of adipophilin that mediates milk lipid formation and secretion. J. Lipid Res. 49: 206-216, 2008
  3. Russell, T.D., Schaack, J., Orlicky, D.J., Palmer, C.A., Chang, B. H-J., Chan, L. and McManaman, J.L. Adipophilin regulates cytoplasmic lipid droplet accumulation and alveolar maturation in differentiating nulliparous mouse mammary glands J. Cell Sci. 124: 3247-3253, 2011.
  4. Chong, B.M., Russell, T.D., Schaack, J., Orlicky, D.J., Reigan, P., Ladinsky, M. and McManaman, J.L. The adipophilin C-terminus is a self-folding membrane binding domain that is important for milk lipid secretion, J. Biol. Chem. 286: 23254-23265, 2011
  5. Wahilg, J.L., Bales, E. Jackman, M.R., Johnson, G.C., McManaman, J.L.*and MacLean, P.S.* Impact of obesity on energy balance and fuel utilization during the metabolic challenge of lactation, Obesity, 20: 65-75, 2011  *,Co-senior authors
  6. McManaman, J.L.*, Bales, E., Orlicky, D.J., Jackman, M., MacLean, P.S., Cain, S., Crunk, A., Mansur, A., Graham, C.E., Bowman, T.A. and Greenberg, A.S.* Plin-2 null mice are protected against diet-induced obesity, adipose inflammation and fatty liver disease.  J. Lipid Res. 54: 1346-1359, 2013  
  7. Orlicky, D.J., Monks, J., Stefanski, A.L. and McManaman, J.L. Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Dispersion.  PLOS ONE 8: e66837, 2013 (PMC3692517).
  8. Crunk, A.E., Monks, J., Murakami, A., Jackman, M., MacLean, P.S., Ladinsky, M., Bales, E.S., Cain, S. Orlicky, D.J. and McManaman, J.L. (2013) Dynamic Regulation of the Hepatic Lipid Droplet Proteome by Diet PLOS ONE 8: e67631, 2013 (PMC3708958) 
  9. Saben, J.L., Bales, E.S., Jackman, M.R., Orlicky, D.J., MacLean, P.S. and McManaman, J.L.  Maternal obesity reduces milk lipid production in lactating mice by inhibiting acetyl-CoA carboxylase and impairing fatty acid synthesis.  PLOS ONE, 9(5) e98066, 2014
  10. Frank, D.N., Bales, E.S., Monks, J. Jackman, M.J., MacLean, P.S., Ir, D., Robertson, C.E., Orlicky, D.J. and McManaman, J.L. Perilipin-2 modulates lipid absorption and microbiome responses in the mouse intestine. PLoS One, 10 (7), e0131944, 2015

NIH PO1-CHD38129, Functional Development of the Mammary Gland

NIH R01HD075285 , Postnatal Actions of Maternal Obesity on Neonatal Metabolic Health