The Department of Medicine at the Anschutz Medical Campus boasts top medical care for patients, as well as fellowships and endowments. The Grand Rounds Schedule includes all events for divisions under the department.

The University of Colorado Cord Blood Bank (UCCBB), a major component of ClinImmune Labs, has a twelve year history of collecting, processing, banking and distributing cord blood for human transplantation. UCCBB is a public cord blood bank, which has consented 10,700 women and banked 6,700 cord blood units, of which more than 500 have been transplanted at 100 different transplant centers in the United States and abroad.

UCCBB is a major provider of Hispanic cord blood units to patients in the United States, Mexico, Central and South America. UCCBB is accredited by AABB and provides cord blood to stem cell investigators in Michigan, New York, Tennessee and Colorado.

UCCBB is managed by immunogeneticists and stem cell transplant physicians with decades of experience in this field. In addition, the parent organization, ClinImmune Labs, provides sophisticated HLA high resolution DNA typing, hematopoietic stem cell and pancreatic islet cell processing, flow cytometry and regulatory oversight to the cord blood bank.

The bank benefits both managerially and financially from an organization that integrates histocompatibility, adult stem cell banking, cellular therapeutics and regulatory affairs. The University of Colorado Cord Blood Bank is unique among banks in the United States in that all of the cord blood units are processed in a Class 1000 cGMP processing facility and managed by CordBloodBankManager® software developed under our guidance and constantly updated to meet our needs.

Please Note: New Checkoff Colorado Opportunity to donate to Statewide Umbilical Cord Blood Collection on Line 39 of Form 104!

Colorado taxpayers can now checkoff a box on their 2009 Form 104 Colorado Individual Tax Return to donate to the ADULT STEM CELLS CURE FUND, which provides resources to encourage and enable new mothers across Colorado to donate their babies' umbilical cord blood to a public cord blood bank. These funds will allow our cord blood bank to collect statewide.

Flow Cytometry and Cell Sorting Facility

The Clinical Immunology Flow Cytometry/Cell Sorting facility is located on the 10th floor of Research 2 on the Anschutz Medical Campus. The facility was established with the specific goal of providing the large community of peer-funded investigators at the University of Colorado Denver and regional biotechnology companies with access to cell analysis technology. The facility is a joint effort by the Division of Allergy & Clinical Immunology and the Colorado Center for AIDS Research (CFAR).The facility provides instrumentation, personnel, and expertise to assist users in multiparametric flow cytometry and fluorescence-activated cell sorting (FACS) for both research and clinical applications. The Flow Cytometry Facility follows Clinical Laboratory Improvement amendments (CLIA) guidelines and is certified by the College of American Pathologists (CAP).

Please note our services, equipment and pricing below:

		Academic Rate	Non-Academic Industry Rate
CELL Analysis
FACScan (1-3 colors)**		$40	$60
FACSCalibar (1-4 colors)**		$50	$75
LSR II (1-13 colors)**		$75	$100
FACSAria (1-13 colors)		$99	$125
Cell Sorting
FACSAria (1-13 colors)*		$125	$150
Data Analysis
FlowJO, Diva, Cell Quest		Free	Free
Training and Consultation		Free	Free

* The price for cell sorting (FACSAria) includes technician time.
* Please add $40/hour to the price of the FACScan, FACSCalibur and LSR II if the samples are run by Flow Lab personnel.

The facility's technical director, Brent Palmer Ph.D., and staff help design experiments, develop and implement new applications as well as collaborate scientifically with users. The staff provide full service flow cytometry assistance, including sample preparation, staining, and data acquisition and analysis. We currently perform immunophenotyping for a number of clinical trials, including CD4/CD8 immunophenotyping for the Adult Clinical Trials Group (ACTG). Click here to review our Transplantation Engraftment Monitoring service that combines sorting with chimerism testing. Please contact Brent Palmer PhD (Brent.Palmer@ucdenver.edu) if you have additional questions about our services.

Information for New Users

The following is a list of facility usage policies. Click here to download the user agreement form, fill out the information at the bottom of the page and return to facility personnel. Samples cannot be analyzed until this form has been signed and returned and a speed type number is provided. To receive access to the scheduling software, Click here to download the request for access to CLINIM/FLOWSHARED.

New users will then need to schedule a time for a training session with the flow laboratory personnel. This training will initially take about 1 hour. To schedule training, please contact Dr. Palmer at 303-724-7203 ( Brent.Palmer@ucdenver.edu). After the initial training, new users will need some supervision and assistance which we will provide until the user and facility staff feel confident that the user can operate the instruments on his or her own. Once approved on the machines, researchers will be able to use the flow cytometers 24 hours a day 7 days a week.

General Information about Flow Cytometry

Flow cytometry is a multi faceted technique for characterizing and delineating cell populations via size, shape, surface and intracellular protein expression, which yields valuable information on both phenotype and cell expression. Flow cytometry is unique in its ability to rapidly analyze and sort thousands of cells per second while simultaneously providing data on distinctive cell populqations resolved at the single cell level. Flow cytometry can be used as a tool to identify novel cell populations based on the expression of cell surface markers through intracellular staining. It can also be used to examine antigen specific T cells, gene expression, or qualify the proliferative capacity of different cell populations. In addition it is a technique that can be performed using any cell sample suspension, e.g., blood cells, collagen digested or disaggregated tissue (e.g. lung, liver, and spleens cells), stem cells and cultured cells.

The BD FACSAria is a cell sorter that is capable of rapid, simultaneous purification of phenotypically distinct populations of cells. Up to four different cell populations can be purified simultaneously from the same sample.

Useful Flow Cytometry Links

• Practical Flow Cytometry Textbook
• Diva Tutorial
• Purdue Website
• BD Spectral Overlap
• H. Maeckers flow site
• Comprehensive list of Fluorochrome excitation and emissions
• Compensation: An informal perspective
• Conjugation of monoclonal antibodies
• International Society for Analytical Cytology (ISAC) Home page
• Antibody resource page (list of antibody suppliers)
• BDbiosciences